Czuper Agnieszka, Gryczynski Ignacy, Kuśba Józef
Faculty of Applied Physics and Mathematics, Gdańsk University of Technology, ul. Narutowicza 11/12, 80-952 Gdańsk, Poland.
J Photochem Photobiol B. 2007 Jun 26;87(3):200-8. doi: 10.1016/j.jphotobiol.2007.04.003. Epub 2007 Apr 24.
Necessary modifications to the expression for the Förster energy transfer rate are discussed when fluorescence decay of the donor in the absence of acceptor is nonexponential. Discrete and continuous models of the nonexponentiality are taken into account. No general solution of the problem is found. It is, however, suggested that in many of the biochemical problems the most appropriate modification of the transfer rate can be that which is based on the assumption of the same constant value of the radiative decay rate for all donor molecules. The effect of the assumed form of the Förster energy transfer rate on the recovered values of the distance distribution and dynamics parameters of some exemplary bichromophoric systems is examined.
当供体在无受体时的荧光衰减为非指数形式时，讨论了对Förster能量转移速率表达式的必要修正。考虑了非指数性的离散和连续模型。未找到该问题的通用解。然而，有人提出，在许多生化问题中，转移速率最恰当的修正可能是基于所有供体分子具有相同辐射衰减率常数这一假设的修正。研究了假定的Förster能量转移速率形式对一些典型双色团体系距离分布和动力学参数恢复值的影响。
