Goins D J, Reynolds R J, Schiff J A, Barnett W E
Biology Division, Oak Ridge National Laboratory, Oak Ridge Graduate School of Biomedical Sciences, Oak Ridge, Tenn. 37820.
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1749-52. doi: 10.1073/pnas.70.6.1749.
An ultraviolet-induced cytoplasmic mutant (G(1)BU) of Euglena gracilis Klebs var. bacillaris Pringsheim is described. G(1)BU, in addition to being golden in color and containing smaller amounts of chlorophyll than wild type when grown in the light, is a regulatory constitutive mutant for the light-inducible chloroplast isoleucine and methionine tRNAs; i.e., these two tRNAs are present in dark-grown G(1)BU cells at approximately the levels present in light-grown wild-type cells. Six other mutants were also examined for normal control of plastid tRNA biosynthesis; all four of the aplastidic mutants (lacking appreciable plastid structures and detectable plastid DNA) are incapable of chloroplast tRNA biosynthesis, whereas two other color mutants that do contain plastids and chloroplast DNA have normal plastid tRNA regulation.
本文描述了纤细裸藻(Euglena gracilis Klebs var. bacillaris Pringsheim)的一种紫外线诱导的细胞质突变体(G(1)BU)。G(1)BU除了颜色为金色且在光照下生长时叶绿素含量比野生型少之外,还是光诱导叶绿体异亮氨酸和甲硫氨酸tRNA的调节组成型突变体;也就是说,这两种tRNA在黑暗中生长的G(1)BU细胞中的含量与在光照下生长的野生型细胞中的含量大致相同。还检查了其他六个突变体对质体tRNA生物合成的正常调控;所有四个无质体突变体(缺乏明显的质体结构和可检测到的质体DNA)都不能进行叶绿体tRNA生物合成,而另外两个含有质体和叶绿体DNA的颜色突变体具有正常的质体tRNA调控。