Reger B J, Fairfield S A, Epler J L, Barnett W E
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1207-13. doi: 10.1073/pnas.67.3.1207.
Light-grown, wild type Euglena gracilis contains two aminoacyl-tRNA synthetases for both phenylalanine and isoleucine. Only one of the two synthetases for each amino acid is found in isolated chloroplasts, as are the light-induced phenylalanine and isoleucine tRNAs. In each case the light-induced chloroplast tRNAs can only be acylated by the chloroplast synthetases. The chloroplast isoleucyl-tRNA synthetase is light-inducible and cannot be detected in dark-grown cells or in cells of the bleached mutant W(3)BUL. The presence of the chloroplast phenylalanyl-tRNA synthetase in W(3)BUL, which contains no chloroplast DNA or structure, indicates that this chloroplast enzyme is synthesized in the cytoplasm and is coded by nuclear genes.
在光照条件下生长的野生型纤细裸藻含有两种分别用于苯丙氨酸和异亮氨酸的氨酰 - tRNA合成酶。在分离的叶绿体中，每种氨基酸的两种合成酶中只有一种存在，光照诱导的苯丙氨酸和异亮氨酸tRNA也是如此。在每种情况下，光照诱导的叶绿体tRNA只能被叶绿体合成酶酰化。叶绿体异亮氨酰 - tRNA合成酶是光诱导型的，在黑暗中生长的细胞或白化突变体W(3)BUL的细胞中无法检测到。在不含有叶绿体DNA或叶绿体结构的W(3)BUL中存在叶绿体苯丙氨酰 - tRNA合成酶，这表明这种叶绿体酶是在细胞质中合成的，由核基因编码。
