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眼虫叶绿体氨酰-tRNA合成酶的转录和翻译位点。

The sites of transcription and translation for Euglena chloroplastic aminoacyl-tRNA synthetases.

作者信息

Hecker L I, Egan J, Reynolds R J, Nix C E, Schiff J A, Barnett W E

出版信息

Proc Natl Acad Sci U S A. 1974 May;71(5):1910-4. doi: 10.1073/pnas.71.5.1910.

DOI:10.1073/pnas.71.5.1910
PMID:4525469
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC388352/
Abstract

We find that cycloheximide completely blocks the light-induced apearance of Euglena chloroplastic aminoacyl-tRNA synthetases in dark-grown cells of Euglena gracilis var. bacillaris. Streptomycin, on the other hand, has no effect on the light-induction of these organellar enzymes. These observations, together with the finding that an aplastidic mutant (strain W(3)BUL, which has neither significant plastid structure nor detectable chloroplast DNA) contains low levels of the chloroplastic synthetases, indicate that the chloroplastic synthetases are transcriptional products of nuclear genes and are translated on cytoplasmic ribosomes prior to compartmentalization within the chloroplasts.

摘要

我们发现，放线菌酮完全阻断了纤细裸藻变种巴氏裸藻黑暗培养细胞中光诱导的叶绿体氨酰 - tRNA合成酶的出现。另一方面，链霉素对这些细胞器酶的光诱导没有影响。这些观察结果，连同无质体突变体（W(3)BUL菌株，既没有明显的质体结构，也没有可检测到的叶绿体DNA）含有低水平叶绿体合成酶这一发现，表明叶绿体合成酶是核基因的转录产物，并且在被分隔到叶绿体内之前在细胞质核糖体上进行翻译。

相似文献

1
The sites of transcription and translation for Euglena chloroplastic aminoacyl-tRNA synthetases.眼虫叶绿体氨酰-tRNA合成酶的转录和翻译位点。
Proc Natl Acad Sci U S A. 1974 May;71(5):1910-4. doi: 10.1073/pnas.71.5.1910.
2
Comments on the translational and transcriptional origin of Euglena chloroplastic aminoacyl-tRNA synthetases.关于眼虫叶绿体氨酰-tRNA合成酶的翻译和转录起源的评论。
Arch Microbiol. 1976 Sep 1;109(3):201-3. doi: 10.1007/BF00446628.
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A reinvestigation of the sites of transcription and translation of Euglena chloroplastic phenylalanyl-tRNA synthetase.对眼虫叶绿体苯丙氨酰 - tRNA合成酶转录和翻译位点的重新研究。
Arch Microbiol. 1975 Oct 27;105(2):117-21. doi: 10.1007/BF00447124.
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Identification and origin of some chloroplast aminoacyl-tRNA synthetases and tRNAs.一些叶绿体氨酰-tRNA合成酶和tRNA的鉴定及起源
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Chloroplastic and cytoplasmic valyl- and leucyl-tRNA synthetases from Euglena gracilis. Comparative study of their structural properties.纤细裸藻的叶绿体和细胞质缬氨酰 - 及亮氨酰 - tRNA合成酶。其结构特性的比较研究。
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Cytoplasmic site synthesis of chloroplast aminoacyl-tRNA synthetases in Euglena gracilis.纤细裸藻中叶绿体氨酰 - tRNA合成酶的细胞质位点合成
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Plant Physiol. 1991 Nov;97(3):1094-102. doi: 10.1104/pp.97.3.1094.
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本文引用的文献

1
Studies on Chloroplast Development and Replication in Euglena: III. A Study of the Site of Synthesis of Alkaline Deoxyribonuclease Induced during Chloroplast Development in Euglena gracilis.眼虫叶绿体发育与复制的研究：III. 纤细眼虫叶绿体发育过程中诱导产生的碱性脱氧核糖核酸酶合成位点的研究
Plant Physiol. 1972 Sep;50(3):391-5. doi: 10.1104/pp.50.3.391.
2
Isolation of Intact Chloroplasts from Euglena gracilis by Zonal Centrifugation.用区带离心法从眼虫中分离完整叶绿体。
Plant Physiol. 1971 Feb;47(2):217-21. doi: 10.1104/pp.47.2.217.
3
Studies of Chloroplast Development in Euglena. V. Pigment Biosynthesis, Photosynthetic Oxygen Evolution and Carbon Dioxide Fixation during Chloroplast Development.眼虫叶绿体发育的研究。V.叶绿体发育过程中的色素生物合成、光合放氧和二氧化碳固定
Plant Physiol. 1964 Mar;39(2):220-6. doi: 10.1104/pp.39.2.220.
4
A Cytoplasmic Regulatory Mutant of Euglena: Constitutivity for the Light-Inducible Chloroplast Transfer RNAs.眼虫的一种细胞质调节突变体：光诱导叶绿体转移RNA的组成型表达
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1749-52. doi: 10.1073/pnas.70.6.1749.
5
STUDIES OF CHLOROPLAST DEVELOPMENT IN EUGLENA. XII. TWO TYPES OF SATELLITE DNA.眼虫叶绿体发育的研究。十二。两种类型的卫星DNA。
J Mol Biol. 1965 Apr;11:769-74. doi: 10.1016/s0022-2836(65)80034-1.
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EVIDENCE FOR DEGENERACY AND AMBIGUITY IN INTERSPECIES AMINOACYL-SRNA FORMATION.种间氨酰基 - tRNA 形成过程中简并性和模糊性的证据。
Proc Natl Acad Sci U S A. 1964 Apr;51(4):642-7. doi: 10.1073/pnas.51.4.642.
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METABOLIC EVENTS DURING THE FORMATION OF A PHOTOSYNTHETIC FROM A NONPHOTOSYNTHETIC CELL.从非光合细胞形成光合细胞过程中的代谢事件。
Brookhaven Symp Biol. 1964 Mar;16:89-108.
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Thermal conversion of nonpriming deoxyribonucleic acid to primer.非引发脱氧核糖核酸向引物的热转化
J Biol Chem. 1959 Oct;234:2733-4.
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A simplified procedure for the preparation of tyrosine and valine-acceptor fractions of yeast "soluble ribonucleic acid".一种制备酵母“可溶性核糖核酸”的酪氨酸和缬氨酸受体组分的简化程序。
J Biol Chem. 1961 Jan;236:200-2.
10
Events surrounding the early development of Euglena chloroplasts. I. Induction by preillumination.围绕眼虫叶绿体早期发育的事件。I. 预照光诱导。
Plant Physiol. 1970 Mar;45(3):339-47. doi: 10.1104/pp.45.3.339.