Erdos G W, Raper K B, Vogen L K
Department of Bacteriology, University of Wisconsin, Madison, Wis. 53706.
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1828-30. doi: 10.1073/pnas.70.6.1828.
Strains of Dictyostelium discoideum were paired in various combinations. Certain pairings resulted in the production of macrocysts, which are thought to be the sexual stage in the cellular slime molds. One selfcompatible strain was found. Other strains produced macrocysts only when paired with an alternate mating type. Two strains were found to form macrycysts when paired with either mating type but not alone or with each other. Several strains did not form macrocysts under any circumstances. Strains were graded according to their mating competency based on the number of macrocysts produced relative to the aggregated myxamoebae observed in the pairings.
以各种组合方式将盘基网柄菌菌株进行配对。某些配对产生了大囊胞,据认为这是细胞黏菌的有性阶段。发现了一种自交亲和菌株。其他菌株只有在与另一种交配型配对时才产生大囊胞。发现有两个菌株在与任何一种交配型配对时都会形成大囊胞,但单独或相互配对时则不会。有几个菌株在任何情况下都不会形成大囊胞。根据配对中观察到的相对于聚集的黏变形体产生的大囊胞数量,对菌株的交配能力进行分级。