Katz E R, Sussman M
Proc Natl Acad Sci U S A. 1972 Feb;69(2):495-8. doi: 10.1073/pnas.69.2.495.
Haploid strains of Dictyostelium discoideum bearing temperature-sensitive mutations have been used to select stable diploid, heterozygotic clones, which arise at low frequency (about 10(-5)). Segregants arise from such diploids at low frequency (about 10(-3)). The diploids were heterozygous for resistance to cycloheximide and were phenotypically sensitive to the drug. Growth of the diploid cells in the presence of cycloheximide automatically selected those segregants bearing the resistant allele, and facilitated examination of the assortment of unselected markers. The combination of the two selective methods provides a workable system of genetic analysis in this species. We have used this method to locate six markers on three different linkage groups.
携带温度敏感突变的盘基网柄菌单倍体菌株已被用于筛选稳定的二倍体杂合克隆，这些克隆出现的频率较低（约10^(-5)）。分离体从这类二倍体中以低频率（约10^(-3)）产生。这些二倍体对环己酰亚胺具有抗性杂合性，并且在表型上对该药物敏感。在环己酰亚胺存在的情况下二倍体细胞的生长自动选择了那些携带抗性等位基因的分离体，并便于对未选择标记的分离进行检测。这两种选择方法的结合提供了该物种可行的遗传分析系统。我们已使用这种方法在三个不同的连锁群上定位了六个标记。
