生长条件对纤细裸藻叶绿体DNA分子数量的影响。
Influence of growth conditions upon the number of chloroplast DNA molecules in Euglena gracilis.
作者信息
Rawson J R, Boerma C
机构信息
Department of Botany, University of Georgia, Athens, Ga. 30602.
出版信息
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2401-4. doi: 10.1073/pnas.73.7.2401.
Abstract
The number of chloroplast DNA molecules in Euglena gracilis cells was measured by determining the shift in the observed second-order rate constant for the reassociation of (125)I-labeled chloroplast DNA in the presence of unlabeled total cell DNA. Cells grown to stationary phase in the dark contained 217 molecules of chloroplast DNA. Cells grown to stationary phase in the light in either heterotrophic or autotrophic medium contained 590 and 1014 chloroplast DNA molecules, respectively. The observed second-order rate constant for the reassociation of (125)I-labeled chloroplast DNA was not significantly altered in the presence of total cell DNA from a heat-bleached mutant, ZHB, which lacks chloroplast DNA. This evidence suggests that there is less than 0.3 of a chloroplast DNA molecule present in the nucleus of Euglena.
摘要
通过测定在未标记的总细胞DNA存在下，（125）I标记的叶绿体DNA重新结合时观察到的二级速率常数的变化，来测量纤细裸藻细胞中叶绿体DNA分子的数量。在黑暗中生长至稳定期的细胞含有217个叶绿体DNA分子。在异养或自养培养基中光照下生长至稳定期的细胞分别含有590和1014个叶绿体DNA分子。在缺乏叶绿体DNA的热漂白突变体ZHB的总细胞DNA存在下，（125）I标记的叶绿体DNA重新结合的观察到的二级速率常数没有显著改变。这一证据表明，裸藻细胞核中存在的叶绿体DNA分子少于0.3个。
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