Powell C A, Zoeten G A
Department of Plant Pathology, University of Wisconsin, Madison, Madison, Wisconsin 53706.
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2919-22. doi: 10.1073/pnas.74.7.2919.
Isolated nuclei from healthy pea plants were primed with pea enation mosaic virus (PEMV), southern bean mosaic virus (SBMV), radish mosaic virus (RdMV), tobacco mosaic virus (TMV), PEMV RNA, SBMV RNA, RdMV RNA, or TMV RNA. RNA replication occurred only with PEMV RNA and not with intact PEMV or any of the other viruses or RNAs, as judged by ensuing actinomycin D-insensitive polymerase activity. Molecular hybridization experiments showed that some of the product of the polymerase was PEMV-specific (-)RNA. The substrate and ionic requirements of this polymerase were the same as those for the RNA-dependent RNA polymerase present in nuclei isolated from PEMV-infected pea plants. No virus particles could be recovered from nuclei primed with PEMV RNA. These results are discussed in relation to the possible mechanism for in vivo infection of pea cells.
从健康豌豆植株中分离出的细胞核,用豌豆耳突花叶病毒(PEMV)、南方菜豆花叶病毒(SBMV)、萝卜花叶病毒(RdMV)、烟草花叶病毒(TMV)、PEMV RNA、SBMV RNA、RdMV RNA或TMV RNA进行引发处理。根据随后放线菌素D不敏感的聚合酶活性判断,RNA复制仅发生在PEMV RNA上,而不是完整的PEMV或任何其他病毒或RNA上。分子杂交实验表明,这种聚合酶的一些产物是PEMV特异性(-)RNA。这种聚合酶的底物和离子需求与从感染PEMV的豌豆植株中分离出的细胞核中存在的依赖RNA的RNA聚合酶相同。用PEMV RNA引发处理的细胞核中未检测到病毒颗粒。结合豌豆细胞体内感染的可能机制对这些结果进行了讨论。