Timmerman-Vaughan Gail, Larsen Richard, Murray Sarah, McPhee Kevin, Coyne Clarice
New Zealand Institute for Plant & Food Research Ltd., Christchurch, New Zealand.
Phytopathology. 2009 Nov;99(11):1281-8. doi: 10.1094/PHYTO-99-11-1281.
Pea enation mosaic virus (PEMV) is an important virus disease of pea. International movement of commercial pea cultivars and germplasm can be problematic due to uncertainty about seed transmission of the viruses responsible for the disease. Whether PEMV is seedborne was assessed by collecting developing seed from infected plants and determining the relative concentrations of the PEMV-1 and PEMV-2 viral genomes using quantitative real-time reverse-transcription polymerase chain reaction. The relative accumulation of PEMV-1 and PEMV-2 was approximately 1,240 and 13,000 times higher, respectively, in leaf than in embryo tissues. Accumulation of PEMV-1 and PEMV-2 RNA was also significantly higher in pod walls and seed coats than in cotyledons or embryo axes. No evidence was obtained for seed transmission of PEMV in pea. Although PEMV-1 and PEMV-2 genomic RNAs were found in developing seed, no PEMV symptoms were observed in the field on more than 50,000 plants from seed derived from PEMV-infected source plants. These data demonstrate that PEMV is seedborne in pea but do not support a previous report that PEMV is seed transmitted. Absence of seed transmission may result from the low abundance of PEMV viral genomes in embryo tissue.
豌豆耳突花叶病毒（PEMV）是豌豆的一种重要病毒病害。由于引发该病害的病毒种子传播情况不确定，商业豌豆品种和种质的国际流通可能会出现问题。通过从受感染植株收集发育中的种子，并使用定量实时逆转录聚合酶链反应测定PEMV - 1和PEMV - 2病毒基因组的相对浓度，来评估PEMV是否通过种子传播。PEMV - 1和PEMV - 2在叶片中的相对积累量分别比胚组织中高约1240倍和13000倍。PEMV - 1和PEMV - 2 RNA在荚壁和种皮中的积累量也显著高于子叶或胚轴。未获得PEMV在豌豆中通过种子传播的证据。虽然在发育中的种子中发现了PEMV - 1和PEMV - 2基因组RNA，但在田间，来自PEMV感染源植株种子的50000多株植株未观察到PEMV症状。这些数据表明PEMV在豌豆中通过种子传播，但不支持之前关于PEMV通过种子传播的报道。种子传播的缺失可能是由于胚组织中PEMV病毒基因组丰度较低所致。
