Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Proc Natl Acad Sci U S A. 1980 May;77(5):2505-9. doi: 10.1073/pnas.77.5.2505.
Using an electrophoretic screening procedure, we have discovered that two species of human serum albumin Mexico occur that are indistinguishable by conventional electrophoretic methods. We suggest that these species be referred to as albumins Mexico-1 and Mexico-2. Isolation and determination of the partial sequence of the cyanogen bromide fragment of albumin Mexico-2 that differs from the corresponding fragment of the common albumin A revealed this variant to arise from at least a glycine/aspartic acid substitution at position 550. This region of the albumin molecule is involved in the binding of the fatty acid, palmitate.
使用电泳筛选程序,我们发现了两种人类血清白蛋白墨西哥变体,这些变体无法通过常规电泳方法区分。我们建议将这些变体称为白蛋白墨西哥-1 和墨西哥-2。分离并测定与常见白蛋白 A 的相应片段不同的白蛋白墨西哥-2 的氰化溴片段的部分序列揭示了这种变体至少是由于第 550 位的甘氨酸/天冬氨酸取代引起的。白蛋白分子的这一区域参与了脂肪酸棕榈酸的结合。