Peters T, Feldhoff R C
Biochemistry. 1975 Jul 29;14(15):3384-91. doi: 10.1021/bi00686a015.
Several fragments of bovine serum albumin have been isolated following limited tryptic hydrolysis and their positions then determined in the bovine serum albumin sequence published by J. R. Brown ((1975), Fed. Proc., Fed. Am. Soc. Exp. Biol. 34, 591). When bovine serum albumin was coupled to palmityl-aminoethylamino-agarose and digested with trypsin, two fragments were obtained: (a) peptide 115-184, containing the highly aromatic disulfide loop 3 of Brown's model, and (b) a larger fragment, residues 377-581, containing disulfide loops 7-9. This fragment constitutes the third of the three domains of the albumin molecule. From bovine serum albumin digested in solution, peptide 115-184 was again obtained, as well as (c) a 39,000-dalton fragment identified as residues 198-581, loops 4-9 of the second and third domains, but with a long, tryptophan-containing segment 204-238 missing from loop 4. The ability to isolate these fragments without cleaving disulfide bridges is partial confirmation of the proposed model of bovine serum albumin as a series of nine independent loops.
经有限的胰蛋白酶水解后，已分离出牛血清白蛋白的几个片段，随后在J. R. 布朗发表的牛血清白蛋白序列中确定了它们的位置（(1975)，《联邦程序》，美国实验生物学会联合会会刊34, 591）。当牛血清白蛋白与棕榈酰氨基乙氨基琼脂糖偶联并用胰蛋白酶消化时，得到了两个片段：(a) 肽段115 - 184，包含布朗模型中的高芳香性二硫键环3；(b) 一个较大的片段，残基377 - 581，包含二硫键环7 - 9。该片段构成白蛋白分子三个结构域中的第三个。从溶液中消化的牛血清白蛋白中，再次获得了肽段115 - 184，以及(c) 一个39,000道尔顿的片段，鉴定为残基198 - 581，即第二和第三结构域的环4 - 9，但环4中缺少一个长的含色氨酸片段204 - 238。在不切断二硫键的情况下分离这些片段的能力部分证实了所提出的牛血清白蛋白模型是由一系列九个独立环组成的。
