Gates and Crellin Laboratories of Chemistry, California Institute of Technology, Pasadena, California 91125.
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3095-7. doi: 10.1073/pnas.77.6.3095.
The acceleration, by a factor of 2500, of the hydrolysis of endo-5-[4'(5')imidazolyl]bicyclo[2.2.1]hept-endo- 2-yl trans-cinnamate by 0.5 M sodium benzoate in 42 mol% dioxane in water can be explained without resort to operation of a "charge-relay" mechanism similar to that often postulated to account for the enzymatic activity of serine proteases. The degree of ionization of 4-methylimidazole and of sodium benzoate in 42 mol% dioxane in water at 60 degrees C have been measured by NMR spectroscopy.
在 60°C 时,通过 NMR 光谱法测量了 42mol%二氧六环中的水、4-甲基咪唑和苯甲酸钠的离解度。0.5M 苯甲酸钠在 42mol%二氧六环中的水,可以将 5-[4'(5')咪唑基]双环[2.2.1]庚-2-endo-2-基反肉桂酸酯的水解加速 2500 倍,这一过程无需采用类似于通常用来解释丝氨酸蛋白酶酶活性的“电荷传递”机制。