酶催化的能量学
Energetics of enzyme catalysis.
作者信息
Warshel A
出版信息
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5250-4. doi: 10.1073/pnas.75.11.5250.
Abstract
Quantitative studies of the energetics of enzymatic reactions and the corresponding reactions in aqueous solutions indicate that charge stabilization is the most important energy contribution in enzyme catalysis. Low electrostatic stabilization in aqueous solutions is shown to be consistent with surprisingly large electrostatic stabilization effects in active sites of enzymes. This is established quantitatively by comparing the relative stabilization of the transition states of the reaction of lysozyme and the corresponding reaction is aqueous solution.
摘要
对酶促反应以及水溶液中相应反应的能量学定量研究表明，电荷稳定化是酶催化过程中最重要的能量贡献。研究表明，水溶液中较低的静电稳定作用与酶活性位点中惊人的大静电稳定效应相一致。通过比较溶菌酶反应过渡态的相对稳定化作用以及水溶液中的相应反应，对此进行了定量验证。
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本文引用的文献
1
Stereochemistry of cooperative effects in haemoglobin.血红蛋白协同效应的立体化学
Nature. 1970 Nov 21;228(5273):726-39. doi: 10.1038/228726a0.
2
Entropic contributions to rate accelerations in enzymic and intramolecular reactions and the chelate effect.熵对酶促反应和分子内反应速率加速的贡献以及螯合效应。
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1678-83. doi: 10.1073/pnas.68.8.1678.
3
Subtilisin; a stereochemical mechanism involving transition-state stabilization.枯草杆菌蛋白酶；一种涉及过渡态稳定化的立体化学机制。
Biochemistry. 1972 Nov 7;11(23):4293-303. doi: 10.1021/bi00773a016.
4
Lysozyme-catalyzed reaction of the N-acetylglucosamine hexasaccharide. Dependence of rate on pH.溶菌酶催化的N-乙酰葡糖胺六糖反应。速率对pH的依赖性。
J Biol Chem. 1973 Jul 10;248(13):4786-92.
5
Physical organic models for the mechanism of lysozyme action.
Adv Enzymol Relat Areas Mol Biol. 1973;37:1-60. doi: 10.1002/9780470122822.ch1.
6
Conformational equilibria and the salt bridge in chymotrypsin.胰凝乳蛋白酶中的构象平衡与盐桥
Cold Spring Harb Symp Quant Biol. 1972;36:71-3. doi: 10.1101/sqb.1972.036.01.012.
7
The mechanisms of hydrolysis of glycosides and their revelance to enzyme-catalysed reactions.
Proc R Soc Lond B Biol Sci. 1967 Apr 18;167(1009):389-401. doi: 10.1098/rspb.1967.0036.
8
Crystallographic studies of the activity of hen egg-white lysozyme.鸡蛋清溶菌酶活性的晶体学研究。
Proc R Soc Lond B Biol Sci. 1967 Apr 18;167(1009):378-88. doi: 10.1098/rspb.1967.0035.
9
Theoretical studies of enzymic reactions: dielectric, electrostatic and steric stabilization of the carbonium ion in the reaction of lysozyme.酶促反应的理论研究：溶菌酶反应中碳正离子的介电、静电和空间稳定作用
J Mol Biol. 1976 May 15;103(2):227-49. doi: 10.1016/0022-2836(76)90311-9.
10
Crystallographic study of the binding of dipeptide inhibitors to thermolysin: implications for the mechanism of catalysis.二肽抑制剂与嗜热菌蛋白酶结合的晶体学研究：对催化机制的启示
Biochemistry. 1977 May 31;16(11):2506-16. doi: 10.1021/bi00630a030.
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