酶催化的能量学
Energetics of enzyme catalysis.
作者信息
Warshel A
出版信息
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5250-4. doi: 10.1073/pnas.75.11.5250.
Abstract
Quantitative studies of the energetics of enzymatic reactions and the corresponding reactions in aqueous solutions indicate that charge stabilization is the most important energy contribution in enzyme catalysis. Low electrostatic stabilization in aqueous solutions is shown to be consistent with surprisingly large electrostatic stabilization effects in active sites of enzymes. This is established quantitatively by comparing the relative stabilization of the transition states of the reaction of lysozyme and the corresponding reaction is aqueous solution.
摘要
对酶促反应以及水溶液中相应反应的能量学定量研究表明，电荷稳定化是酶催化过程中最重要的能量贡献。研究表明，水溶液中较低的静电稳定作用与酶活性位点中惊人的大静电稳定效应相一致。通过比较溶菌酶反应过渡态的相对稳定化作用以及水溶液中的相应反应，对此进行了定量验证。
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