在一个完全合成的血红素蛋白模型中对 CO 结合的空间位阻控制。

Steric control of CO binding in a totally synthetic heme protein model.

机构信息

Department of Chemistry, The Ohio State University, Columbus, Ohio 43210.

出版信息

Proc Natl Acad Sci U S A. 1981 Oct;78(10):5919-23. doi: 10.1073/pnas.78.10.5919.

DOI:10.1073/pnas.78.10.5919

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC348946/

Abstract

A family of totally synthetic models for the carbon monoxide adducts of heme proteins has been synthesized and applied to the elucidation of the role of steric effects in the relative detoxification of carbon monoxide. The complexes are designed such that a sheltered void of controllable dimensions encompasses the CO binding site. Systematic variations in the available space for the iron-bound CO produce a wide range of equilibrium binding constants (K(CO)). An x-ray structure determination of a CO adduct complex having a crowded CO site reveals that the Fe-C identical withO linkage is bent, and further, the distortion involves both displacement of the Fe-C vector from the normal to the N(4) plane and bending of the Fe-C-O angle.

摘要

已经合成了一系列完全合成的模型，用于模拟血红素蛋白的一氧化碳加合物，并将其应用于阐明空间位阻效应对一氧化碳相对解毒作用的影响。这些配合物的设计使得一个可控制尺寸的遮蔽空洞包围着 CO 结合位点。对于铁结合的 CO 来说，可用空间的系统变化会产生广泛的平衡结合常数（K(CO)）。对具有拥挤 CO 位点的 CO 加合物配合物的 X 射线结构测定表明，Fe-C 键与 O 的连接是弯曲的，并且这种扭曲涉及 Fe-C 向量从正常到 N(4)平面的位移以及 Fe-C-O 角度的弯曲。

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