高分子质量叶绿素脱辅基-apomyoglobin 复合物的巨大圆二色性。
Giant circular dichroism of high molecular weight chlorophyllide-apomyoglobin complexes.
机构信息
Battelle Memorial Institute, Columbus Laboratories, Columbus, Ohio 43201.
出版信息
Proc Natl Acad Sci U S A. 1982 Jan;79(2):400-2. doi: 10.1073/pnas.79.2.400.
Abstract
Chlorophyllide a and the apoprotein of myoglobin (Mb) spontaneously form three types of complex. The M (M(r) approximately 3 x 10(5)) and H (M(r) >/= 4 x 10(6)) complexes, but not the L (M(r) approximately 1.7 x 10(4)), display a circular dichroism (CD) spectrum that is highly red-shifted, nonconservative, and very intense-characteristics shared by the CD spectra of reaction center complexes from purple photosynthetic bacteria. At its 710-nm peak, the H complex CD spectrum has a larger magnitude, 0.06 differential absorbance per unit total absorbance, than has been reported for chlorophyll in any medium.
摘要
叶绿素 a 和肌红蛋白（Mb）的脱辅基蛋白自发形成三种类型的复合物。M（Mr 约 3 x 10(5)) 和 H（Mr >= 4 x 10(6)) 复合物，但不是 L（Mr 约 1.7 x 10(4)) 复合物，显示出高度红移、非保守且非常强烈的圆二色性（CD）光谱特征，与来自紫色光合细菌的反应中心复合物的 CD 光谱特征相同。在其 710nm 峰处，H 复合物 CD 光谱的幅度更大，单位总吸收的差分吸光度为 0.06，比任何介质中报道的叶绿素都要大。
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