脂质体介导的烟草花叶病毒 RNA 转染烟草原生质体：监测脂质体-原生质体相互作用的敏感方法。
Liposome-mediated delivery of tobacco mosaic virus RNA into tobacco protoplasts: A sensitive assay for monitoring liposome-protoplast interactions.
机构信息
Monsanto Company, St. Louis, Missouri 63167.
出版信息
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1859-63. doi: 10.1073/pnas.79.6.1859.
Abstract
Tobacco mosaic virus (TMV) RNA was encapsulated in large, unilamellar phospholipid vesicles (liposomes), and the encapsulated TMV RNA was shown to be infectious when incubated with tobacco protoplasts under appropriate conditions. Maximal virus production in protoplasts was observed after their incubation with TMV RNA entrapped in phosphatidylserine/cholesterol liposomes. Infection was dependent on the presence of polyalcohols in the incubation mixture. Other parameters, such as the extent of vesicle binding, the cell-induced leakage of vesicle contents, and the degree of liposome toxicity were shown to be important in determining the efficiency of infectivity. Liposome-mediated delivery offers an efficient and reproducible method for introducing RNA into plant protoplasts.
摘要
烟草花叶病毒（TMV）的 RNA 被包裹在大型单层磷脂囊泡（脂质体）中，当在适当的条件下与烟草原生质体共孵育时，包裹的 TMV RNA 显示出感染性。在用磷脂酰丝氨酸/胆固醇脂质体包裹的 TMV RNA 孵育原生质体后，观察到最大的病毒产量。感染依赖于孵育混合物中聚醇的存在。其他参数，如囊泡结合的程度、囊泡内容物的细胞诱导泄漏以及脂质体毒性的程度，对于确定感染效率都很重要。脂质体介导的传递为将 RNA 导入植物原生质体提供了一种高效且可重复的方法。
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1
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2
Evaluation of parameters in the isolation of viable protoplasts from cultured tobacco cells.培养烟草细胞中原生质体分离中参数的评估。
Plant Physiol. 1974 Dec;54(6):936-44. doi: 10.1104/pp.54.6.936.
3
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
4
Phosphorus assay in column chromatography.柱色谱法中的磷测定
J Biol Chem. 1959 Mar;234(3):466-8.
5
Fluorescence studies on the mechanism of liposome-cell interactions in vitro.体外脂质体与细胞相互作用机制的荧光研究。
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6
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7
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8
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