Department of Biochemistry, Oklahoma State University, 74078, Stillwater, Oklahoma, USA.
Plant Cell Rep. 1985 Apr;4(2):58-62. doi: 10.1007/BF00269206.
The infectivity of reverse phase evaporation (REV) liposome-encapsidated cauliflower mosaic virus (CaMV) to turnip protoplasts was tested. The uptake of neutral or negative liposomes was stimulated by polyethylene glycol (PEG), while high levels of uptake of positive liposomes were obtained both in the absence and presence of PEG. The delivery of the vesicle contents to the protoplasts paralleled the uptake of liposomes. CaMV delivered to turnip protoplasts was degraded during the early period of culture. No increase in the amount of CaMV DNA could be detected on longer periods of culture. In contrast, when protoplasts were evacuolated prior to addition of REV liposomes, an increase in the amount of CaMV DNA was noted after some initial degradation of the input DNA.
反向蒸发（REV）脂质体包裹的花椰菜花叶病毒（CaMV）对萝卜原生质体的感染力进行了测试。聚乙二醇（PEG）可刺激中性或负电脂质体的摄取，而在有无 PEG 的情况下，均可获得大量正电脂质体的摄取。囊泡内容物递送至原生质体与脂质体的摄取相平行。在培养的早期，递送至萝卜原生质体的 CaMV 被降解。在较长的培养时间内，无法检测到 CaMV DNA 量的增加。相比之下，当原生质体在添加 REV 脂质体之前进行排空处理时，在输入 DNA 最初降解后，可观察到 CaMV DNA 量的增加。
