大脂质体包裹的豇豆花叶病毒（CCMV）RNA 感染豇豆叶肉原生质体。

Infection of cowpea mesophyll protoplasts with cowpea chlorotic mottle virus (CCMV) RNA encapsulated in large liposomes.

机构信息

Program in Genetics and Cell Biology, Molecular Genetics, Heald Hall, Washington State University, 99164-4350, Pullman, WA, USA.

出版信息

Plant Cell Rep. 1983 Feb;2(1):43-6. doi: 10.1007/BF00269234.

DOI:10.1007/BF00269234

PMID:24257855

Abstract

It has been demonstrated that cowpea chlorotic mottle virus RNA encapsulated in phosphatidyl serine/cholesterol reverse evaporation vesicles (REV) could infect cowpea mesophyll protoplasts under conditions known to enhance liposome-protoplast interactions. Positively charged phosphatidylcholine/stearylamine multilamellar liposomes did not deliver functional CCMV RNA despite their very high nucleic acid trapping capacity and their high affinity for protoplasts.

摘要

已经证明，在已知可以增强脂质体-原生质体相互作用的条件下，包被在磷脂酰丝氨酸/胆固醇逆向蒸发囊泡（REV）中的豇豆花叶病毒 RNA 可以感染豇豆叶肉原生质体。尽管带正电荷的磷脂酰胆碱/硬脂胺多层脂质体具有很高的核酸捕获能力和对原生质体的高亲和力，但它们并不能传递功能性的 CCMV RNA。