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大脂质体包裹的豇豆花叶病毒(CCMV)RNA 感染豇豆叶肉原生质体。

Infection of cowpea mesophyll protoplasts with cowpea chlorotic mottle virus (CCMV) RNA encapsulated in large liposomes.

机构信息

Program in Genetics and Cell Biology, Molecular Genetics, Heald Hall, Washington State University, 99164-4350, Pullman, WA, USA.

出版信息

Plant Cell Rep. 1983 Feb;2(1):43-6. doi: 10.1007/BF00269234.

DOI:10.1007/BF00269234
PMID:24257855
Abstract

It has been demonstrated that cowpea chlorotic mottle virus RNA encapsulated in phosphatidyl serine/cholesterol reverse evaporation vesicles (REV) could infect cowpea mesophyll protoplasts under conditions known to enhance liposome-protoplast interactions. Positively charged phosphatidylcholine/stearylamine multilamellar liposomes did not deliver functional CCMV RNA despite their very high nucleic acid trapping capacity and their high affinity for protoplasts.

摘要

已经证明,在已知可以增强脂质体-原生质体相互作用的条件下,包被在磷脂酰丝氨酸/胆固醇逆向蒸发囊泡(REV)中的豇豆花叶病毒 RNA 可以感染豇豆叶肉原生质体。尽管带正电荷的磷脂酰胆碱/硬脂胺多层脂质体具有很高的核酸捕获能力和对原生质体的高亲和力,但它们并不能传递功能性的 CCMV RNA。

相似文献

1
Infection of cowpea mesophyll protoplasts with cowpea chlorotic mottle virus (CCMV) RNA encapsulated in large liposomes.大脂质体包裹的豇豆花叶病毒(CCMV)RNA 感染豇豆叶肉原生质体。
Plant Cell Rep. 1983 Feb;2(1):43-6. doi: 10.1007/BF00269234.
2
Binding of cowpea chlorotic mottle virus to cowpea protoplasts and relation of binding to virus entry and infection.豇豆褪绿斑驳病毒与豇豆原生质体的结合以及结合与病毒进入和感染的关系。
Virology. 1988 May;164(1):91-8. doi: 10.1016/0042-6822(88)90623-x.
3
Double infection of tobacco protoplasts with brome mosaic virus and cowpea chlorotic mottle virus.烟草原生质体被雀麦花叶病毒和豇豆褪绿斑驳病毒双重感染。
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Small-Scale Isolation of Viral RNA-Dependent RNA Polymerase from Protoplasts Inoculated with In Vitro Transcripts.从体外转录本接种的原生质体中分离病毒 RNA 依赖性 RNA 聚合酶的小规模方法。
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Infectious in vitro transcripts from cowpea chlorotic mottle virus cDNA clones and exchange of individual RNA components with brome mosaic virus.来自豇豆花叶病毒cDNA克隆的体外感染性转录本以及单个RNA组分与雀麦花叶病毒的交换。
J Virol. 1988 Oct;62(10):3581-8. doi: 10.1128/JVI.62.10.3581-3588.1988.
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Inoculation of protoplasts with viruses by electroporation.通过电穿孔法用病毒对原生质体进行接种。
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Site-specific single amino acid changes to Lys or Arg in the central region of the movement protein of a hybrid bromovirus are required for adaptation to a nonhost.杂种雀麦花叶病毒运动蛋白中心区域特定位点的单个氨基酸向赖氨酸或精氨酸的变化是适应非寄主所必需的。
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Substantial portions of the 5' and intercistronic noncoding regions of cowpea chlorotic mottle virus RNA3 are dispensable for systemic infection but influence viral competitiveness and infection pathology.豇豆褪绿斑驳病毒RNA3的5'端和基因间非编码区的大部分区域对于系统感染来说并非必需,但会影响病毒的竞争力和感染病理学。
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A simple technique for separation of Cowpea chlorotic mottle virus from Cucumber mosaic virus in natural mixed infections.一种在自然混合感染中从黄瓜花叶病毒中分离豇豆花叶病毒的简单技术。
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Bromovirus movement protein conditions for the host specificity of virus movement through the vascular system and affects pathogenicity in cowpea.雀麦花叶病毒运动蛋白决定了病毒通过维管系统运动的宿主特异性,并影响豇豆的致病性。
Mol Plant Microbe Interact. 2000 Nov;13(11):1195-203. doi: 10.1094/MPMI.2000.13.11.1195.

引用本文的文献

1
Infection of evacuolated turnip protoplasts with liposome-packaged cauliflower mosaic virus.用脂质体包被的花椰菜花叶病毒感染去壁的萝卜原生质体。
Plant Cell Rep. 1985 Apr;4(2):58-62. doi: 10.1007/BF00269206.

本文引用的文献

1
A method for high-frequency intergeneric fusion of plant protoplasts.一种植物原生质体高频属间融合的方法。
Planta. 1974 Dec;115(4):355-67. doi: 10.1007/BF00388618.
2
Purification and viability determinations of plant protoplasts.植物原生质体的纯化和活力测定。
Planta. 1976 Jan;128(3):213-6. doi: 10.1007/BF00393231.
3
Liposome-mediated delivery of DNA to carrot protoplasts.脂质体介导的 DNA 向胡萝卜原生质体的递送。
Planta. 1981 Oct;153(1):90-4. doi: 10.1007/BF00385322.
4
Liposome-mediated infection of plant protoplasts with tobacco mosaic virus RNA.脂质体介导烟草花叶病毒RNA对植物原生质体的感染。
Virology. 1981 Sep;113(2):752-60. doi: 10.1016/0042-6822(81)90203-8.
5
Transfer of Liposome-Sequestering Plasmid DNA into Daucus carota Protoplasts.脂质体捕获质粒 DNA 向胡萝卜原生质体的转移。
Plant Physiol. 1981 Nov;68(5):1027-30. doi: 10.1104/pp.68.5.1027.
6
Liposome-mediated delivery of tobacco mosaic virus RNA into tobacco protoplasts: A sensitive assay for monitoring liposome-protoplast interactions.脂质体介导的烟草花叶病毒 RNA 转染烟草原生质体:监测脂质体-原生质体相互作用的敏感方法。
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1859-63. doi: 10.1073/pnas.79.6.1859.
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Liposomes with a large trapping capacity prepared by freezing and thawing of sonicated phospholipid mixtures.通过对超声处理的磷脂混合物进行冷冻和解冻制备的具有大包裹容量的脂质体。
Arch Biochem Biophys. 1981 Nov;212(1):186-94. doi: 10.1016/0003-9861(81)90358-1.
8
Fluorescent antibody staining of tobacco mosaic virus antigen in tobacco mesophyll protoplasts.烟草叶肉原生质体中烟草花叶病毒抗原的荧光抗体染色。
Virology. 1969 Jul;38(3):497-9. doi: 10.1016/0042-6822(69)90167-6.
9
The effect of high pH and calcium on tobacco leaf protoplast fusion.高pH值和钙对烟草叶原生质体融合的影响。
Z Naturforsch C. 1973 Nov-Dec;28(11):737-41. doi: 10.1515/znc-1973-11-1215.
10
The use of an abrasive in the isolation of cowpea leaf protoplasts which support the multiplication of cowpea mosaic virus.在豇豆叶原生质体分离中使用研磨剂,豇豆叶原生质体可支持豇豆花叶病毒的增殖。
Virology. 1975 Mar;64(1):272-6. doi: 10.1016/0042-6822(75)90099-9.