Chemistry Department, University of Wisconsin, Madison, Wisconsin 53706.
Proc Natl Acad Sci U S A. 1982 Mar;79(6):2128-32. doi: 10.1073/pnas.79.6.2128.
An extremely short instrumental response function for a single-photon-counting system has been obtained by using a low-jitter photomultiplier tube, fast amplification of the single photoelectron pulse from this photomultiplier, a constant fraction discriminator with a wide bandwidth input, and a stable reference timing signal. This synchronously mode-locked laser system has been shown to be capable of measuring the fluorescence lifetimes of compounds from 66 psec to 4.5 nsec. The estimated error in these determinations is 6% or 10 psec, whichever is greater. This apparatus has proved itself to be superior to the previously used nitrogen flashlamp apparatus for the determination of subnanosecond lifetimes. The validity of using deconvolution to determine lifetimes shorter than the instrumental response function has been verified.
通过使用具有低抖动的光电倍增管、对该光电倍增管的单光电子脉冲进行快速放大、具有宽频带输入的恒比定时甄别器以及稳定的参考定时信号，获得了单光子计数系统的极短仪器响应函数。该同步锁模激光系统已被证明能够测量从 66 皮秒到 4.5 纳秒的化合物的荧光寿命。这些测定的估计误差为 6%或 10 皮秒，以较大者为准。该仪器已被证明优于先前用于测定亚纳秒寿命的氮闪光灯仪器。已经验证了使用反卷积来确定比仪器响应函数更短的寿命的有效性。
