Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska 68583-0722.
Proc Natl Acad Sci U S A. 1982 Jun;79(12):3867-71. doi: 10.1073/pnas.79.12.3867.
We previously reported that isolation of symbiotic Chlorella-like algae from the Florida strain of Hydra viridis induced replication of a virus (designated HVCV-1) in the algae. We now report that isolation of symbiotic Chlorella-like algae from four other sources of green hydra and one source of the protozoan Paramecium bursaria also induced virus synthesis. Algae from one of these hydra contained a virus identical to HVCV-1 (based on its rate of sedimentation, buoyant density, reaction to HVCV-1 antiserum, and DNA restriction fragments) whereas algae from the other three hydra contained another similar, but distinct, virus (designated HVCV-2). The virus from the paramecium algae (designated PBCV-1) was distinct from both HVCV-1 and HVCV-2. The symbiotic algae in the hydra could also be distinguished ultrastructurally. Chloroplasts of both algae that produced HVCV-1 lacked a pyrenoid whereas chloroplasts of the other three symbiotic algae contained pyrenoids. Since all symbiotic eukaryotic algae we have examined have had virus, a potential viral role in symbiosis is suggested.
我们之前曾报道过，从佛罗里达州的 Hydra viridis 分离共生的类似绿藻的藻类，会诱导藻类中病毒（称为 HVCV-1）的复制。我们现在报告说，从其他四个绿螅和一个原生动物 Paramecium bursaria 的来源分离共生的类似绿藻，也会诱导病毒的合成。从其中一种绿螅中分离出来的藻类含有与 HVCV-1 完全相同的病毒（基于其沉降率、浮力密度、对 HVCV-1 抗血清的反应以及 DNA 限制片段），而从其他三种绿螅中分离出来的藻类含有另一种类似但不同的病毒（称为 HVCV-2）。从草履虫藻类（称为 PBCV-1）中分离出的病毒与 HVCV-1 和 HVCV-2 均不同。绿螅中的共生藻类在超微结构上也可以区分开来。产生 HVCV-1 的两种藻类的叶绿体都缺乏淀粉核，而其他三种共生藻类的叶绿体则含有淀粉核。由于我们检查过的所有共生真核藻类都有病毒，因此病毒在共生中的潜在作用是被暗示的。
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