从与绿水螅共生的细胞内绿藻中分离并鉴定一种病毒。

Isolation and characterization of a virus from the intracellular green alga symbiotic with Hydra viridis.

作者信息

Van Etten J L, Meints R H, Burbank D E, Kuczmarski D, Cuppels D A, Lane L C

机构信息

Department of Plant Pathology, University of Nebraska at Lincoln, Lincoln, Nebraska 68583, USA.

出版信息

Virology. 1981 Sep;113(2):704-11. doi: 10.1016/0042-6822(81)90199-9.

DOI:10.1016/0042-6822(81)90199-9

PMID:18635089

Abstract

The isolation and characterization of a virus (designated HVCV) from the symbiotic Chlorella-like green alga present in Hydra viridis (Florida strain) are described. The large polyhedral viral particle sedimented at ca. 2600 S in sucrose density gradients and had a buoyant density in CsCl of 1.295 g/ml. The virus contained at least 19 polypeptides (MW range, 10,300 to 82,000) and the major polypeptide (MW, 46,000) was a glycoprotein. The viral genome consisted of a double-stranded DNA with a buoyant density in CsCl of 1.7114 g/ml (52% GC) and a molecular weight of ca. 136 x 10(6).

摘要

本文描述了从绿水螅（佛罗里达株）中存在的共生小球藻样绿藻中分离和鉴定一种病毒（命名为HVCV）的过程。这种大型多面体病毒粒子在蔗糖密度梯度中约2600 S处沉降，在CsCl中的浮力密度为1.295 g/ml。该病毒含有至少19种多肽（分子量范围为10,300至82,000），主要多肽（分子量为46,000）是一种糖蛋白。病毒基因组由双链DNA组成，在CsCl中的浮力密度为1.7114 g/ml（GC含量为52%），分子量约为136×10⁶ 。