Department of Biophysics, University of California, Berkeley, California 94720.
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5494-8. doi: 10.1073/pnas.79.18.5494.
photosystems I and II and the cytochrome b(6)-f complex. This system shows an absolute dependence on the presence of all three protein complexes for NADP reduction, in addition to plastocyanin, ferredoxin, and ferredoxin-NADP reductase. The reconstructed system was found to be sensitive to low concentrations of known inhibitors of noncyclic electron transport. Depletion of the Rieske iron-sulfur center and bound plastoquinone from the cytochrome b(6)-f complex resulted in an inhibition of the photoreduction of NADP.
光系统 I 和 II 以及细胞色素 b6-f 复合物,完成了从水到 NADP 的光合非循环电子传递的重建。除了质体蓝素、铁氧还蛋白和铁氧还蛋白-NADP 还原酶外,该系统对于 NADP 的还原还表现出对所有三种蛋白复合物存在的绝对依赖性。该重建系统被发现对已知的非循环电子传递抑制剂的低浓度敏感。细胞色素 b6-f 复合物中 Rieske 铁-硫中心和结合的质体醌的耗竭导致 NADP 的光还原受到抑制。