Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado, 80309.
Plant Physiol. 1985 May;78(1):199-202. doi: 10.1104/pp.78.1.199.
We have visualized directly the distribution of the cytochrome b(6)/f and coupling factor ATP synthetase complexes in thylakoid membranes of embedded, thin-sectioned, intact chloroplasts by using rabbit antibodies directed against each complex, followed by ferritin-conjugated goat anti- (rabbit immunoglobulin G) antibodies. The labeling patterns indicate that in spinach (Spinacia oleracea) chloroplasts the cytochrome b(6)/f complex is distributed laterally throughout both stacked grana and unstacked stroma membrane regions, whereas the coupling factor ATP synthetase complex is found exclusively in stroma thylakoids and in the marginal and end membranes of grana.
我们通过使用针对每个复合物的兔抗体，然后使用铁蛋白结合的山羊抗（兔免疫球蛋白 G）抗体，直接可视化了嵌入的、薄切片的完整叶绿体类囊体膜中细胞色素 b(6)/f 和偶联因子 ATP 合成酶复合物的分布。标记模式表明，在菠菜（Spinacia oleracea）叶绿体中，细胞色素 b(6)/f 复合物分布在堆叠的粒状片层和未堆叠的基质膜区域的两侧，而偶联因子 ATP 合成酶复合物仅存在于基质类囊体和粒状片层的边缘和末端膜中。
