柑橘脆皮层病毒无细胞体系合成的特性。
Properties of a cell-free system for synthesis of citrus exocortis viroid.
机构信息
Department of Plant Pathology and Cell Interaction Research Group, University of California, Riverside, California 92521.
出版信息
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6285-8. doi: 10.1073/pnas.79.20.6285.
Partially purified nuclei from citrus exocortis viroid (CEV)-infected Gynura aurantiaca are able to synthesize linear and circular viroid molecules. Pretreatment of the nuclei with actinomycin D or deoxyribonuclease did not affect viroid synthesis, whereas the synthesis of other cellular RNAs was severely reduced. These observations support the essential role of CEV complementary RNA sequences in viroid replication. However, when alpha-amanitin was included in this in vitro synthesis system, CEV replication was markedly reduced by concentrations of 10 nM or greater. Taken together, these data support the proposition that viroid synthesis is catalyzed by a DNA-dependent RNA polymerase acting on a RNA template.
从感染柑橘外果皮脆缩病毒（CEV）的 Gynura aurantiaca 中部分纯化的核能够合成线性和环形的病毒分子。用放线菌素 D 或脱氧核糖核酸酶预处理核不会影响病毒的合成，而其他细胞 RNA 的合成则严重减少。这些观察结果支持 CEV 互补 RNA 序列在病毒复制中的重要作用。然而，当α-鹅膏蕈碱被包含在这个体外合成系统中时，CEV 的复制会被 10 nM 或更高浓度的 α-鹅膏蕈碱显著减少。总的来说，这些数据支持这样的假设，即病毒的合成是由一种作用于 RNA 模板的 DNA 依赖性 RNA 聚合酶催化的。
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