小麦胚芽中一种新型RNA连接酶形成2'-磷酸单酯、3',5'-磷酸二酯键。

Formation of a 2'-phosphomonoester, 3',5'-phosphodiester linkage by a novel RNA ligase in wheat germ.

作者信息

Konarska M, Filipowicz W, Domdey H, Gross H J

出版信息

Nature. 1981;293(5828):112-6. doi: 10.1038/293112a0.

Abstract

Extracts of wheat germ contain an RNA ligase activity that catalyses the conversation of linear polyribonucleotides into covalently closed circles. The newly formed 3',5'-phosphodiester bridge is resistant to alkali and a number of ribonucleases. This unusual stability is due to the presence of a phosphoryl group esterified to the 2'-position of the 3'-nucleotide joined. This is the first demonstration of an RNA ligase in higher plants and of a natural 2'-phosphomonoester, 3',5'-phosphodiester linkage.

摘要

小麦胚芽提取物含有一种RNA连接酶活性，可催化线性多聚核糖核苷酸转化为共价闭合环。新形成的3',5'-磷酸二酯桥对碱和多种核糖核酸酶具有抗性。这种不寻常的稳定性归因于在连接的3'-核苷酸的2'-位酯化的磷酰基的存在。这是高等植物中RNA连接酶以及天然2'-磷酸单酯、3',5'-磷酸二酯键的首次证明。