Department of Genetics, University of California, Berkeley, California 94720.
Proc Natl Acad Sci U S A. 1983 May;80(9):2661-5. doi: 10.1073/pnas.80.9.2661.
There are several biochemical differences between the callus and the embryos of carrot culture. Callus tissue produces callus-specific proteins and a conditioning factor that is necessary for the synthesis of callus-specific proteins. By contrast, embryos produce embryo-specific proteins [Sung, Z. R. & Okimoto, R. (1981) Proc. Natl. Acad. Sci. USA 78, 3683-3687] and develop the capability to inactivate cycloheximide [Sung, Z. R., Lazar, G. J. & Dudits, D. (1981) Plant Physiol. 68, 261-264]. A mutant, WCH105, that can inactivate cycloheximide in the callus as well as in the embryos produces the embryo-specific proteins instead of the callus-specific proteins and fails to produce the conditioning factor by the callus tissue. Callus tissues also produce a conditioning factor for callus growth. This factor is not the same as the conditioning factor for the synthesis of the callus-specific proteins, as WCH105 can grow as callus. The existence of WCH105 demonstrates that the callus-specific and embryo-specific traits are coordinately regulated, but in an opposite manner. A common mechanism apparently activates one set and inactivates the other set of functions. WCH105 seems to be impaired in this mechanism.
愈伤组织和胡萝卜培养胚胎之间存在一些生化差异。愈伤组织产生愈伤组织特异性蛋白和条件因子，这是合成愈伤组织特异性蛋白所必需的。相比之下，胚胎产生胚胎特异性蛋白[Sung，Z.R.和 Okimoto，R.（1981）Proc. Natl. Acad. Sci. USA 78，3683-3687]，并具有使环己酰亚胺失活的能力[Sung，Z.R.，Lazar，G.J.和 Dudits，D.（1981）Plant Physiol. 68，261-264]。一种名为 WCH105 的突变体可以使环己酰亚胺在愈伤组织和胚胎中失活，产生胚胎特异性蛋白而不是愈伤组织特异性蛋白，并且不能由愈伤组织产生条件因子。愈伤组织还产生一种用于愈伤组织生长的条件因子。这种因子与合成愈伤组织特异性蛋白的条件因子不同，因为 WCH105 可以作为愈伤组织生长。WCH105 的存在表明，愈伤组织特异性和胚胎特异性性状是协调调控的，但方式相反。一种共同的机制显然激活了一组功能，而使另一组功能失活。WCH105 似乎在这个机制中受损。
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