Department of Genetics, University of California, Berkeley, California 94720.
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3683-7. doi: 10.1073/pnas.78.6.3683.
The translational profile of cultured carrot cells (callus) was compared with that of the somatic embryos derived from them. The two tissues synthesize almost the same number and kinds of polypeptides except for two "embryonic" proteins. These were found in the somatic embryos but were nearly undetectable in the callus. Both embryo development and the production of embryonic proteins were induced by the same trigger (transfer of the callus to fresh medium) and were suppressed by the same factor (2,4-dichlorophenoxyacetic acid). But the appearance and disappearance of the proteins occurred several days prior to embryo formation and to the conversion of embryo to callus, respectively. Carrot cell lines incapable of embryogenesis could not synthesize the embryonic proteins. These findings indicate that the embryonic proteins play a key role in the process of embryo development. The function of these proteins is presently unknown; however, they can serve as early developmental markers for studying the mechanisms underlying somatic embryogeny in plants.
培养的胡萝卜细胞（愈伤组织）的翻译谱与由其衍生的体细胞胚进行了比较。除了两种“胚胎”蛋白外，这两种组织合成的多肽数量和种类几乎相同。这些在体细胞胚中发现，但在愈伤组织中几乎检测不到。胚胎发育和胚胎蛋白的产生都由相同的触发因素（将愈伤组织转移到新鲜培养基）诱导，并且都受到相同的因素（2,4-二氯苯氧乙酸）的抑制。但是，蛋白质的出现和消失分别发生在胚胎形成之前几天和胚胎向愈伤组织转化之前几天。不能进行胚胎发生的胡萝卜细胞系不能合成胚胎蛋白。这些发现表明，胚胎蛋白在胚胎发育过程中起着关键作用。这些蛋白质的功能目前尚不清楚；但是，它们可以作为研究植物体细胞胚胎发生机制的早期发育标记物。
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