Department of Genetics, North Carolina State University, Raleigh, North Carolina 27650.
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4055-9. doi: 10.1073/pnas.80.13.4055.
Mitochondria from the S male-sterile cytoplasm (cms-S) of maize contain two plasmid-like DNAs, S-1 and S-2, that appear to be prominently involved with the cytoplasmic male sterility trait. The complete nucleotide sequence of the S-2 DNA molecule was determined by the chain termination method. The linear S-2 DNA molecule contains 5,452 base pairs and is terminated by exact 208-base-pair inverted repetitions. Two large open reading frames were identified in the S-2 DNA, suggesting the possibility of protein-encoding genes. The nucleotide sequence of the S-2 termini are discussed with regard to models proposed for the replication of linear DNA molecules.
玉米 S 细胞质雄性不育(cms-S)线粒体中含有两种类似质粒的 DNA,S-1 和 S-2,它们似乎与细胞质雄性不育性状密切相关。通过链终止法测定了 S-2DNA 分子的完整核苷酸序列。线性 S-2DNA 分子包含 5452 个碱基对,由精确的 208 碱基对反向重复序列终止。在 S-2DNA 中鉴定出两个大的开放阅读框,表明可能存在编码蛋白质的基因。还讨论了 S-2 末端的核苷酸序列,以及针对线性 DNA 分子复制提出的模型。