Départment de Biochimie, Pavillon Vachon, Université Laval, G1K 7P4, Québec, Québec, Canada.
Plant Mol Biol. 1986 Sep;6(5):313-9. doi: 10.1007/BF00034938.
The unicellular green alga Chlamydomonas moewusii contains small DNA species of unknown cellular location. We report that the most abundant of these DNAs, here designated low-molecular-weight DNA (LMW DNA), is a linear molecule of 5.9 kilobase pairs (kbp). Southern blot hybridization and restriction enzyme analysis revealed that the LMW DNA sequence also exists as an integrated sequence in a discrete region of the chloroplast genome. We have confirmed earlier reports that small DNA species related to the LMW DNA are absent from Chlamydomonas eugametos, an alga which is interfertile with C. moewusii. In the C. eugametos chloroplast genome we found only remnants of the LMW DNA sequence.
单细胞绿藻衣藻（Chlamydomonas moewusii）含有位置未知的小 DNA 物种。我们报告说，这些 DNA 中最丰富的一种，这里称为低分子量 DNA（LMW DNA），是一种 5.9 千碱基对（kbp）的线性分子。Southern 印迹杂交和限制性内切酶分析表明，LMW DNA 序列也作为一个整合序列存在于叶绿体基因组的一个离散区域。我们已经证实了早期的报告，即与 LMW DNA 相关的小 DNA 物种不存在于衣藻（Chlamydomonas eugametos）中，衣藻与衣藻（C. moewusii）是可杂交的。在 C. eugametos 叶绿体基因组中，我们只发现了 LMW DNA 序列的残余物。
