Department of Crop Science, North Carolina State University, Raleigh, NC 27695-7620.
Proc Natl Acad Sci U S A. 1987 Aug;84(15):5374-8. doi: 10.1073/pnas.84.15.5374.
Mitochondrial DNA in the T male-sterile cytoplasm (cms-T) of maize contains an open reading frame (ORF 13) associated with the T type of sterility. Antibodies raised to a chemically synthesized oligopeptide corresponding to ORF 13 were used to establish the expression of a 13-kDa protein from this reading frame. The 13-kDa polypeptide is synthesized uniquely in cms-T maize and purifies with the membrane fraction of T mitochondria. We assign the symbol urf13-T to designate this mitochondrial gene. Presence of the nuclear restorer gene Rf1 in cms-T plants results in a decrease in abundance of 13-kDa protein and alteration in the transcripts of urf13-T.
玉米 T 雄性不育细胞质(cms-T)中的线粒体 DNA 含有一个与 T 型不育性相关的开放阅读框(ORF13)。针对对应于 ORF13 的化学合成寡肽产生的抗体被用于确定来自该阅读框的 13kDa 蛋白的表达。这个 13kDa 多肽仅在 cms-T 玉米中合成,并与 T 线粒体的膜部分一起纯化。我们将这个线粒体基因命名为 urf13-T。在 cms-T 植物中核恢复基因 Rf1 的存在导致 13kDa 蛋白丰度的降低和 urf13-T 转录本的改变。