Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Apartado postal 565-A, Cuernavaca, Morelos, Mexico.
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1170-4. doi: 10.1073/pnas.82.4.1170.
The complete coding sequence of the nitrogenase reductase gene (nifH) is present in three different regions of a Rhizobium phaseoli symbiotic plasmid. Homology between two of the regions containing nifH coding sequences extends over 5 kilobases. These in turn share 1.3 kilobases of homology with the third region. The nucleotide sequences of the three nitrogenase reductase genes were found to be identical. Site-directed insertion mutagenesis indicated that none of the three genes is indispensable for nitrogen fixation during symbiosis with Phaseolus vulgaris. This implies that at least two of the reiterated genes can be functionally expressed.
固氮酶还原酶基因（nifH）的完整编码序列存在于根瘤菌属豌豆共生质粒的三个不同区域。含有 nifH 编码序列的两个区域之间的同源性超过 5 千碱基。这两个区域又与第三个区域共享 1.3 千碱基的同源性。发现三个固氮酶还原酶基因的核苷酸序列完全相同。定点插入突变表明，这三个基因在与菜豆共生固氮过程中都不是不可或缺的。这意味着至少有两个重复基因可以进行功能表达。
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