Institute of Molecular Genetics, University of Heidelberg, Heidelberg, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1985 Sep;82(17):5795-9. doi: 10.1073/pnas.82.17.5795.
Screening a cDNA library of 2- to 3-day-old flies with poly(A)(+) RNA from male and female flies, we were able to isolate a small number of clones hybridizing preferentially with RNA from female flies. Four cDNA clones, derived from a single mRNA species, proved to be highly abundant in female flies. When we screened a genomic library with the longest cDNA, we obtained two genomic clones, F1 and F2; F1 was a direct copy of the cDNA and F2 was obtained by cross-hybridization. A detailed analysis of these genomic clones revealed two independent genes coding for proteins of 50 kDa that are >90% homologous. RNA analysis with gene-specific probes from the 3' untranslated region showed an expression of F1 in all stages of development with a 5- to 10-fold overexpression of this RNA in female flies compared with males. In contrast to F1, F2 is mainly expressed in late pupae and is expressed only at low levels in adult flies.
我们以雌雄蝇的多聚腺苷酸（poly(A)+）RNA为探针，从 2-3 日龄的雄蝇和雌蝇 cDNA 文库中筛选，分离到一些优先与雌蝇 RNA 杂交的克隆。有 4 个 cDNA 克隆来源于单一的 mRNA 种类，在雌蝇中高度丰富。当我们用最长的 cDNA 筛选基因组文库时，我们得到了两个基因组克隆，F1 和 F2；F1 是 cDNA 的直接拷贝，F2 是通过交叉杂交得到的。对这些基因组克隆的详细分析表明，它们编码两个独立的蛋白基因，分子量为 50kDa，同源性>90%。来自 3'非翻译区的基因特异性探针的 RNA 分析显示，F1 在发育的所有阶段都有表达，与雄蝇相比，这种 RNA 在雌蝇中的表达水平高出 5-10 倍。与 F1 相反，F2 主要在晚期蛹中表达，在成蝇中的表达水平较低。
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