European Molecular Biology Laboratory, Postfach 10.2209, D-6900 Heidelberg, FRG.
EMBO J. 1983;2(6):927-34. doi: 10.1002/j.1460-2075.1983.tb01523.x.
Fragments from the 3B-3C region of the Drosophila X chromosomes were microdissected from salivary gland squashes and their DNA was cloned by the method developed by Scalenghe et al. (1981). These clones were used as starting points for a chromosome walk which covers 200 kb including bands 3B2 to 3C2. A number of deletion breakpoints were mapped on the cloned DNA allowing the localisation of several genes in the 3B region. The white locus in particular was isolated by microcloning the w insertion site. Two transcripts, of 2 and 2.4 kb, respectively, arise from the white region and its vicinity.
从果蝇 X 染色体的 3B-3C 区切下的片段从唾液腺刮片中提取出来，并通过 Scalenghe 等人（1981）开发的方法对其 DNA 进行克隆。这些克隆被用作染色体行走的起点，涵盖了包括 3B2 到 3C2 带在内的 200kb 区域。许多缺失断点被定位在克隆的 DNA 上，从而可以在 3B 区域定位几个基因。特别是通过微克隆 w 插入位点，分离了白色基因座。来自白色区域及其附近的两个转录物分别为 2kb 和 2.4kb。
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