Autonomously replicating RNA in mitochondria of maize plants with S-type cytoplasm.

Mitochondria isolated from maize plants with S-type male-sterile cytoplasms are capable of synthesizing four species of RNA at concentrations of actinomycin D that eliminate all DNA-directed RNA synthesis. No RNA synthesis occurs under the same conditions with mitochondria from plants possessing normal (N) cytoplasm or with other subcellular fractions from plants with S cytoplasm. The actinomycin D-resistant RNA synthesis occurs within the mitochondria since the labeling of these species is unaffected by inclusion of RNase in the incubation medium and since they become completely sensitive to RNase upon lysis of the mitochondria with low concentrations of Triton X-100. Two of the actinomycin D-resistant products are double stranded. These are 2850 and 900 base pairs in length, whereas the remaining two are 2150 and 850 bases. The synthesis of all four RNAs occurs in at least five different accessions of S cytoplasm, suggesting it is a general feature of S mitochondria. The double-stranded RNAs show homology to single-stranded S mitochondrial RNA but not to N mitochondrial RNA. Our observations indicate that the replication of these RNAs occurs independently of mtDNA and that they thus represent a novel type of inheritable element in organelles, an RNA plasmid.