Nassuth A, Alblas F, van der Geest A J, Bol J F
Virology. 1983 Apr 30;126(2):517-24. doi: 10.1016/s0042-6822(83)80009-9.
Actinomycin D, added early after inoculation, reduces the production of infectious alfalfa mosaic virus in cowpea protoplasts by 90%. This reduction was associated with an inhibition of viral minus-strand and plus-strand RNA synthesis, suggesting the involvement of host factors in these processes. Coat protein production was less affected by the drug. Addition of cycloheximide throughout the growth cycle resulted in an immediate cessation of coat protein production and an enhanced degradation of viral RNA. This degradation obscured possible effects of the drug on viral RNA synthesis.
接种后早期添加放线菌素D可使豇豆原生质体中传染性苜蓿花叶病毒的产量降低90%。这种降低与病毒负链和正链RNA合成的抑制有关，表明宿主因子参与了这些过程。衣壳蛋白的产生受该药物的影响较小。在整个生长周期中添加环己酰亚胺会导致衣壳蛋白的产生立即停止，并增强病毒RNA的降解。这种降解掩盖了该药物对病毒RNA合成可能产生的影响。
