分离的酵母线粒体中的RNA合成
RNA synthesis in isolated yeast mitochondria.
作者信息
Groot G S, van Harten-Loosbroek N, van Ommen G J, Pijst H L
出版信息
Nucleic Acids Res. 1981 Dec 11;9(23):6369-77. doi: 10.1093/nar/9.23.6369.
Isolated yeast mitochondria incorporate added UTP into RNA. Amongst the products formed are the two rRNAs, 4S RNA and several components presumed to be mRNAs. In omega+ strains (containing an intervening sequence in the 21S rRNA gene) besides mature 21S rRNA a transcript could be detected still containing nucleotide sequences transcribed from this intervening sequence. In omega- strains (not containing this intervening sequence) also a longer form of the 21S rRNA could be observed. These results suggest that isolated yeast mitochondria are capable of carrying out RNA synthesis and processing, including splicing.
分离出的酵母线粒体可将添加的尿苷三磷酸（UTP）掺入RNA中。形成的产物包括两种核糖体RNA（rRNA）、4S RNA以及几种被认为是信使RNA（mRNA）的组分。在ω⁺菌株（21S rRNA基因中含有一个间隔序列）中，除了成熟的21S rRNA外，还能检测到一种转录本，其仍含有从该间隔序列转录而来的核苷酸序列。在ω⁻菌株（不含有此间隔序列）中，也能观察到21S rRNA的一种较长形式。这些结果表明，分离出的酵母线粒体能够进行RNA合成及加工，包括剪接。
Zotero 插件