Department of Genetics, University of California, Berkeley, CA 94720.
Proc Natl Acad Sci U S A. 1987 Apr;84(7):1906-10. doi: 10.1073/pnas.84.7.1906.
Genes specifically induced during somatic embryogenesis may play key roles in plant embryo development. An antiserum against an extract of carrot somatic embryos revealed a few rare antigens induced at the onset of embryogenesis. Through differential immunoadsorption techniques, we purified antibodies against the embryo-specific antigens and probed a phage lambda gt11 library of cDNA from carrot somatic embryos. This paper describes three distinct cDNA clones that hybridize to embryo-specific RNAs. Monospecific antibodies, purified by affinity to the recombinant phage fusion proteins, confirm that the cloned cDNAs encode unique embryo-specific peptide antigens. One 50-kDa protein correlates with embryogenic ability in cultures of other plant species, including cereals.
在体细胞胚胎发生过程中特异性诱导的基因可能在植物胚胎发育中起关键作用。针对胡萝卜体细胞胚胎提取物的抗血清揭示了在胚胎发生开始时诱导的一些罕见抗原。通过差异免疫吸附技术，我们纯化了针对胚胎特异性抗原的抗体，并探测了来自胡萝卜体细胞胚胎的噬菌体 λgt11 cDNA 文库。本文描述了三个与胚胎特异性 RNA 杂交的独特 cDNA 克隆。通过对重组噬菌体融合蛋白的亲和力进行纯化的单特异性抗体，证实所克隆的 cDNA 编码独特的胚胎特异性肽抗原。一种 50kDa 的蛋白质与包括谷物在内的其他植物物种培养中的胚胎发生能力相关。
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