Sakata J, Uyeda K
Department of Veterans Affairs Medical Center, University of Texas Southwestern Medical Center, Dallas 75216.
Biochem Biophys Res Commun. 1991 Oct 31;180(2):470-4. doi: 10.1016/s0006-291x(05)81088-5.
Occurrence of two isozymic forms of fructose 6-P, 2-kinase: fructose 2,6-bisphosphatase in bovine heart was investigated by transcribing mRNAs and amplifying the cDNAs with polymerase chain reactions. Analysis of the PCR products revealed 1.7 Kb and 1.5 Kb DNAs, and the determination of their nucleotide sequences showed that these DNAs are identical except for the lack of 180 base pairs near the 3' of the bovine heart enzyme DNA previously reported (6). This missing nucleotide sequence encodes Asn451-Gln510 and contains the phosphorylation sites for cAMP dependent protein kinase and protein kinase C.
通过转录mRNA并用聚合酶链反应扩增cDNA，研究了牛心中果糖6 - 磷酸，2 - 激酶：果糖2,6 - 二磷酸酶两种同工酶形式的存在情况。对PCR产物的分析显示有1.7 Kb和1.5 Kb的DNA，对其核苷酸序列的测定表明，除了在先前报道的牛心酶DNA 3'端附近缺少180个碱基对外，这些DNA是相同的(6)。这个缺失的核苷酸序列编码Asn451 - Gln510，并包含cAMP依赖性蛋白激酶和蛋白激酶C的磷酸化位点。
