牛心脏中6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶新亚型的克隆与表达

Cloning and expression of novel isoforms of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from bovine heart.

作者信息

Vidal H, Crepin K M, Rider M H, Hue L, Rousseau G G

机构信息

Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.

出版信息

FEBS Lett. 1993 Sep 20;330(3):329-33. doi: 10.1016/0014-5793(93)80898-5.

DOI:10.1016/0014-5793(93)80898-5

PMID:8397106

Abstract

Distinct 6-phosphofructo-2-kinase (PFK-2)/fructose 2,6-bisphosphatase (FBPase-2) cDNAs were cloned from bovine heart, showing that PFK-2/FBPase-2 gene B, which contains 16 exons, codes for at least five mRNAs. Three of them (B1, B2, B4) could encode the 58,000-M(r) isozyme. In B2 mRNA, exon 15 encodes four more residues than in B1. In B4 mRNA, exon 15 encodes six more residues than in B1, but exon 16 (20 residues) is missing. B3 mRNA corresponds to the 54,000-M(r) isozyme. It lacks exon 15 and also differs from the other mRNAs in the 5' noncoding region. B5 mRNA encodes a truncated form. When expressed in E. coli, the recombinant isoforms corresponding to all these mRNAs except B5 exhibited PFK-2 activity.

摘要

从牛心脏中克隆出了不同的6-磷酸果糖-2-激酶（PFK-2）/果糖2,6-二磷酸酶（FBPase-2）cDNA，这表明含有16个外显子的PFK-2/FBPase-2基因B编码至少五种mRNA。其中三种（B1、B2、B4）可编码58,000相对分子质量的同工酶。在B2 mRNA中，外显子15编码的氨基酸比B1多四个。在B4 mRNA中，外显子15编码的氨基酸比B1多六个，但外显子16（20个氨基酸）缺失。B3 mRNA对应54,000相对分子质量的同工酶。它缺少外显子15，并且在5'非编码区也与其他mRNA不同。B5 mRNA编码一种截短形式。当在大肠杆菌中表达时，除B5外，与所有这些mRNA对应的重组同工型均表现出PFK-2活性。