Department of Biology, University of North Carolina, Chapel Hill, NC 27599.
Proc Natl Acad Sci U S A. 1989 Aug;86(16):6153-6. doi: 10.1073/pnas.86.16.6153.
The photoaffinity labeling agent 5-azidoindole-3-acetic acid, an analog of the endogenous plant hormone indole-3-acetic acid (an auxin), was used to identify indole-3-acetic acid-binding proteins in maize. Two peptides with subunit molecular masses of 24 and 22 kilodaltons are specifically labeled in a saturable manner. Both peptides are slightly acidic and behave as dimers under nondenaturing conditions. The possibility that one of these peptides is the auxin receptor that mediates cell elongation in maize is discussed.
photoaffinity labeling agent 5-azidoindole-3-acetic acid，一种内源性植物激素吲哚-3-乙酸（生长素）的类似物，被用于鉴定玉米中的吲哚-3-乙酸结合蛋白。两种亚基分子量为 24 和 22 千道尔顿的肽以可饱和的方式被特异性标记。这两种肽都略带酸性，在非变性条件下表现为二聚体。讨论了其中一种肽是否是介导玉米细胞伸长的生长素受体。
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