Windsor Rebecca C, Johnson Lynelle R, Sykes Jane E, Drazenovich Tracy L, Leutenegger Christian M, De Cock Hilde E V
Veterinary Medical Teaching Hospital, University of California, Davis, USA.
J Vet Intern Med. 2006 Mar-Apr;20(2):250-6. doi: 10.1892/0891-6640(2006)20[250:mdomin]2.0.co;2.
Lymphoplasmacytic rhinitis (LPR) is a common histologic finding in dogs with chronic nasal disease; however, potential etiologies of this disorder have not been examined. We investigated the hypothesis that specific microbes contribute to clinical disease in dogs with LPR. Paraffin-embedded nasal biopsies were obtained from 19 dogs with LPR, 10 dogs with nasal neoplasia, and 10 dogs with nasal aspergillosis. Nucleic acids were extracted from paraffin blocks, and real-time quantitative polymerase chain reaction (PCR) was employed for detection of target genes for bacterial and fungal DNA, canine adenovirus 2 (CAV-2), parainfluenza virus 3 (PI-3), Chlamydial Chlamydophila spp., and Bartonella spp. Conventional PCR was used for detection of Mycoplasma spp. Statistical analysis was performed using the Mann-Whitney U-test for nonparametric data, and significance was set at P < 0.05. DNA or RNA for CAV-2, PI-3, Bartonella, Mycoplasma, and Chlamydophila was not detected in any nasal biopsy. DNA loads for bacterial DNA did not differ among disease groups. Detection of fungal DNA in nasal biopsies was highest in dogs with aspergillosis (P < 0.0001); however, nasal biopsies of LPR dogs also displayed higher fungal DNA levels than samples from dogs with nasal neoplasia (P = 0.016). Detection of high levels of fungal DNA in nasal biopsies of dogs with LPR suggests that fungal organisms may be causally associated with the inflammation observed, although the possibility of entrapment or accumulation of fungi in the nasal cavity due to chronic inflammation cannot be excluded. Further investigations are required to elucidate the underlying etiopathogenesis of LPR.
淋巴浆细胞性鼻炎（LPR）是患有慢性鼻腔疾病犬类常见的组织学表现；然而，该病症的潜在病因尚未得到研究。我们调查了特定微生物导致LPR犬临床疾病的假说。从19只患有LPR的犬、10只患有鼻腔肿瘤的犬和10只患有鼻曲霉菌病的犬获取石蜡包埋的鼻腔活检组织。从石蜡块中提取核酸，并采用实时定量聚合酶链反应（PCR）检测细菌和真菌DNA、犬腺病毒2型（CAV - 2）、副流感病毒3型（PI - 3）、衣原体属和巴尔通体属的靶基因。采用常规PCR检测支原体属。使用曼 - 惠特尼U检验对非参数数据进行统计分析，显著性设定为P < 0.05。在任何鼻腔活检组织中均未检测到CAV - 2、PI - 3、巴尔通体、支原体和衣原体的DNA或RNA。疾病组之间细菌DNA的载量无差异。鼻曲霉菌病犬的鼻腔活检组织中真菌DNA的检测率最高（P < 0.0001）；然而，LPR犬的鼻腔活检组织中真菌DNA水平也高于鼻腔肿瘤犬的样本（P = 0.016）。LPR犬鼻腔活检组织中检测到高水平真菌DNA表明真菌可能与观察到的炎症存在因果关系，尽管不能排除由于慢性炎症导致真菌在鼻腔内滞留或积聚的可能性。需要进一步研究以阐明LPR的潜在病因发病机制。
