Mercier E, Peters I R, Billen F, Battaille G, Clercx C, Day M J, Peeters D
Department of Veterinary Clinical Sciences, Division of Companion Animal Internal Medicine, Faculty of Veterinary Medicine, University of Liège, Sart Tilman, 4000, Liège, Belgium.
J Small Anim Pract. 2013 Apr;54(4):179-83. doi: 10.1111/jsap.12049.
To evaluate the possible role of Alternaria and Cladosporium species in the pathogenesis of canine lymphoplasmacytic rhinitis by comparing the amount of specific fungal DNA in nasal mucosal biopsies between dogs without nasal neoplasia and those with lymphoplasmacytic rhinitis or nasal neoplasia.
Quantitative real-time polymerase chain reaction (qPCR) assays detecting DNA from Alternaria and Cladosporium fungi were applied to nasal mucosal biopsies collected from dogs with lymphoplasmacytic rhinitis (n = 8), dogs with nasal neoplasia (n = 10) and control animals (n = 10). A copy number for each sample was calculated using a standard curve of known copy number and differences amongst groups were assessed using Kruskal-Wallis tests.
No significant difference was found between the groups. Low levels of Alternaria DNA (10-100 copies/PCR) were detected in one sample; very low levels of DNA (<10 copies/qPCR) were detected in 6 samples, and 21 samples were negative. Low levels of Cladosporium DNA were detected in 2 samples; very low levels of DNA in 18; and 8 were negative.
Results of this study reveal that Alternaria and Cladosporium species are part of the canine nasal flora, and that these fungi are probably not involved in the pathogenesis of lymphoplasmacytic rhinitis.
通过比较无鼻肿瘤犬与患淋巴细胞浆细胞性鼻炎或鼻肿瘤犬鼻黏膜活检组织中特定真菌DNA的含量，评估链格孢属和枝孢属真菌在犬淋巴细胞浆细胞性鼻炎发病机制中可能的作用。
采用定量实时聚合酶链反应（qPCR）检测法，对从患淋巴细胞浆细胞性鼻炎的犬（n = 8）、患鼻肿瘤的犬（n = 10）和对照动物（n = 10）采集的鼻黏膜活检组织进行链格孢属和枝孢属真菌DNA检测。使用已知拷贝数的标准曲线计算每个样本的拷贝数，并采用Kruskal-Wallis检验评估各组之间的差异。
各组之间未发现显著差异。在1个样本中检测到低水平的链格孢属DNA（10 - 100拷贝/PCR）；在6个样本中检测到极低水平的DNA（<10拷贝/qPCR），21个样本为阴性。在2个样本中检测到低水平的枝孢属DNA；18个样本中检测到极低水平的DNA；8个样本为阴性。
本研究结果表明，链格孢属和枝孢属真菌是犬鼻内菌群的一部分，这些真菌可能不参与淋巴细胞浆细胞性鼻炎的发病机制。
