Couto C G, Lara A, Iazbik M C, Brooks M B
Veterinary Teaching Hospital, Department of Veterinary Clinical Sciences, College of Veterinary Medicine, OSU Comprehensive Cancer Center,The Ohio State University, Columbus, USA.
J Vet Intern Med. 2006 Mar-Apr;20(2):365-70. doi: 10.1892/0891-6640(2006)20[365:eopaua]2.0.co;2.
Veterinarians involved in Greyhound rescue have anecdotally observed that 10-15% of Greyhounds bleed profusely after simple surgical procedures. In most patients, platelet counts and hemostasis profiles are normal; therefore, it is possible that these dogs have platelet dysfunction. The PFA-100 is a novel point-of-care platelet function analyzer that has recently been evaluated as a rapid method to assess platelet function in dogs. The objectives of this study were to characterize platelet function in a group of healthy Greyhounds by means of the PFA-100. Blood samples were collected from the jugular vein from 30 healthy Greyhounds. CBC, biochemical profile, PFA-100 assay with collagen/epinephrine (COL-EPI) and collagen/ adenosindiphosphate (COL-ADP), plasma von Willebrand factor antigen concentration (vWF:Ag), and vWF collagen-binding assay (vWF:CBA) were performed. PFA-100 closure times (CTs) with COL/ADP ranged from 63 to 92 seconds (mean +/- SD, 74.7 +/- 7.9 seconds) and with COL/EPI from 87 to 238 seconds (138 +/- 41 seconds); vWF: Ag ranged from 22 to 120% (87.52 +/- 25.5%) and vWF: CBA ranged from 36 to 102% (77.4 +/- 17.3%); and platelet counts ranged from 147 to 265 x 10(9)/L (194.6 +/- 31.64 x 10(9)/L). Greyhound CTs were significantly shorter than CTs in a mixed population of 50 healthy non-Greyhound dogs, in which the COL/ADP CTs ranged from 61 to 172 seconds (mean +/- SD, 87 +/- 21.6 seconds), and the COL/ EPI CTs ranged from 81 to 300 seconds (mean +/- SD, 183 +/- 67.6 seconds; P = 0.005 for COL/ADP CT; P = 0.001 for COL/ EPI CT). Also, platelet counts were significantly lower (P = 0.001) and packed cell volume was significantly higher (P = 0.001) in the Greyhound than in the non-Greyhound group. The PFA-100 is a reproducible method that can be used in the clinical setting to assess platelet function in Greyhounds; however, normal CTs in healthy Greyhounds are shorter than in other breeds. The results obtained in this study will be used to screen for abnormal platelet function in Greyhounds with postoperative bleeding.
参与灵缇犬救助工作的兽医们凭经验观察到，10%至15%的灵缇犬在简单外科手术后会大量出血。在大多数这类病犬中，血小板计数和止血指标均正常；因此，这些犬只可能存在血小板功能障碍。PFA - 100是一种新型即时检验血小板功能分析仪，最近被评估为一种评估犬只血小板功能的快速方法。本研究的目的是通过PFA - 100对一组健康灵缇犬的血小板功能进行特征描述。从30只健康灵缇犬的颈静脉采集血样。进行了全血细胞计数、生化指标检测、使用胶原/肾上腺素（COL - EPI）和胶原/二磷酸腺苷（COL - ADP）的PFA - 100检测、血浆血管性血友病因子抗原浓度（vWF:Ag）以及vWF胶原结合检测（vWF:CBA）。COL/ADP的PFA - 100封闭时间（CT）范围为63至92秒（平均值±标准差，74.7±7.9秒），COL/EPI的CT范围为87至238秒（138±41秒）；vWF:Ag范围为22%至120%（87.52±25.5%），vWF:CBA范围为36%至102%（77.4±17.3%）；血小板计数范围为147至265×10⁹/L（194.6±31.64×10⁹/L）。灵缇犬的CT明显短于50只健康非灵缇犬的混合群体，其中COL/ADP的CT范围为61至172秒（平均值±标准差为87±21.6秒），COL/EPI的CT范围为81至300秒（平均值±标准差为183±67.6秒；COL/ADP CT的P = 0.005；COL/EPI CT的P = 0.001）。此外，灵缇犬的血小板计数显著更低（P = 0.001），而红细胞压积显著更高（P = 0.001）。PFA - 100是一种可重复的方法，可用于临床环境中评估灵缇犬的血小板功能；然而，健康灵缇犬的正常CT比其他品种的要短。本研究所得结果将用于筛查术后出血的灵缇犬是否存在异常血小板功能。
