Park J S, Rader J S, Wu T C, Laimins L A, Currie J L, Kurman R J, Shah K V
Johns Hopkins Medical Institutions, Baltimore, Maryland 21205.
Gynecol Oncol. 1991 Sep;42(3):250-5. doi: 10.1016/0090-8258(91)90354-8.
Specific human papillomavirus (HPV) types are strongly associated with intraepithelial neoplasia and invasive cancer of the cervix. In contrast, the role of HPVs in the pathogenesis of invasive carcinoma of the vulva is poorly understood. We have employed in situ hybridization for the detection of subgenomic transcripts in four vulvar specimens to elucidate the role of HPV type 16 in the development and progression of vulvar cancer. These analyses revealed that the transcripts of the E6-E7 region were more abundant than those of the L1-L2 region in vulvar neoplastic tissues. The transcripts from early and late region of HPV-16 continued to increase with the differentiation of the epithelial cells in both the warty and the basaloid types of vulvar precancerous lesions. This pattern persisted in invasive warty carcinoma but not in basaloid invasive carcinoma; the transcripts in basaloid carcinoma were distributed in an even and discrete pattern. In contrast to earlier studies, L1-L2-region transcripts, as well as viral capsid protein, were detected in focal areas of well-differentiated cells of invasive warty carcinoma. These findings suggest that expression of HPV-16 is regulated by the degree of cellular differentiation.
特定的人乳头瘤病毒（HPV）类型与宫颈上皮内瘤变和浸润性癌密切相关。相比之下，HPV在外阴浸润性癌发病机制中的作用却知之甚少。我们采用原位杂交技术检测了四个外阴标本中的亚基因组转录本，以阐明16型HPV在外阴癌发生发展中的作用。这些分析表明，在外阴肿瘤组织中，E6 - E7区域的转录本比L1 - L2区域的更为丰富。在疣状和基底样型外阴癌前病变中，HPV - 16早期和晚期区域的转录本随着上皮细胞的分化而持续增加。这种模式在浸润性疣状癌中持续存在，但在基底样浸润性癌中则不然；基底样癌中的转录本呈均匀且离散的分布。与早期研究不同的是，在浸润性疣状癌高分化细胞的局部区域检测到了L1 - L2区域转录本以及病毒衣壳蛋白。这些发现表明，HPV - 16的表达受细胞分化程度的调控。
