Pilotti S, Rotola A, D'Amato L, Di Luca D, Shah K V, Cassai E, Rilke F
Division of Anatomical Pathology and Cytology, Istituto Nazionale Tumori, Milano, Italy.
Mod Pathol. 1990 Jul;3(4):442-8.
Ten cases of intraepithelial carcinoma, five with Bowenoid features and five with early invasion, and ten cases of invasive vulvar carcinoma were examined by in situ hybridization and Southern blot analysis using DNA probes for human papillomavirus (HPV) types 6, 11, 16, 18 and 31. HPV DNA was detected in 90% of the intraepithelial cases and in 10% of the invasive cases. All positive cases showed the presence of DNA of HPV type 16. The cases with intraepithelial lesions revealed a strong correlation between the presence of HPV type 16 DNA, cigarette smoking habit, other potential cofactors such as herpes simplex (HSV) DNA sequences and the use of contraceptive drugs, and clinicopathologic features of Bowen's type in situ squamous cell carcinoma. Similar associations were not observed among the cases with invasive disease. While HPV-16 is associated with differentiated Bowenoid type vulvar intraepithelial neoplasia, which appears to be the most common form of early carcinoma of the vulva, the same association was not seen with respect to advanced vulvar invasive squamous cell carcinoma.
采用针对人乳头瘤病毒（HPV）6、11、16、18和31型的DNA探针，通过原位杂交和Southern印迹分析，对10例上皮内癌（其中5例具有鲍温样特征，5例有早期浸润）以及10例浸润性外阴癌进行了检测。在上皮内癌病例中，90%检测到HPV DNA，而在浸润性病例中这一比例为10%。所有阳性病例均显示存在HPV 16型DNA。上皮内病变病例显示，HPV 16型DNA的存在、吸烟习惯、其他潜在辅助因素（如单纯疱疹病毒（HSV）DNA序列）以及避孕药的使用，与鲍温型原位鳞状细胞癌的临床病理特征之间存在密切关联。在浸润性疾病病例中未观察到类似关联。虽然HPV - 16与分化型鲍温样外阴上皮内瘤变相关，而这似乎是外阴早期癌最常见的形式，但对于晚期外阴浸润性鳞状细胞癌，未发现相同的关联。
