柘树根的保肝化合物对他克林诱导的Hep G2细胞毒性的作用

Hepatoprotective compounds of the roots of Cudrania tricuspidata on tacrine-induced cytotoxicity in Hep G2 cells.

作者信息

An Ren-Bo, Sohn Dong-Hwan, Kim Youn-Chul

机构信息

College of Pharmacy, Wonkwang University, Iksan, Korea.

出版信息

Biol Pharm Bull. 2006 Apr;29(4):838-40. doi: 10.1248/bpb.29.838.

DOI:10.1248/bpb.29.838

PMID:16595932

Abstract

The bioassay-guided fractionation of the MeOH extract of the root barks of Cudrania tricuspidata furnished three hepatoprotective compounds, gerontoxanthone A (2), cudraflavone B (4), gericudranin E (5), together with four prenylated xanthones, cudraxanthone B (3), isocudraxanthone K (6), cudraxanthone C (7), cudraxanthone H (8), and a prenylated flavanone, euchrestaflavanone C (1). Compounds 4 and 5 showed significant hepatoprotective effects with the EC50 values of 37.39+/-0.4 and 39.87+/-0.7 microM, respectively, and compound 2 showed moderate hepatoprotective effect with an EC50 value of 125.9+/-1.5 microM, against tacrine-induced cytotoxicity in Hep G2 cells. Silybin as positive control showed an EC50 value of 84.76+/-0.5 microM. Isocudraxanthone K (6) is a new compound.

摘要

对柘树根皮甲醇提取物进行生物活性导向的分离，得到了三种具有肝保护作用的化合物，即老鹳草黄烷酮A（2）、柘黄酮B（4）、老鹳柘黄素E（5），以及四种异戊烯基取代的呫吨酮，即柘黄烷酮B（3）、异柘黄烷酮K（6）、柘黄烷酮C（7）、柘黄烷酮H（8），还有一种异戊烯基取代的黄烷酮，即宽叶羽扇豆黄烷酮C（1）。化合物4和5对他克林诱导的Hep G2细胞毒性具有显著的肝保护作用，其半数有效浓度（EC50）值分别为37.39±0.4和39.87±0.7微摩尔，化合物2具有中等程度的肝保护作用，EC50值为125.9±1.5微摩尔。水飞蓟宾作为阳性对照，其EC50值为84.76±0.5微摩尔。异柘黄烷酮K（6）是一种新化合物。