Miyamoto Shingo, Kohno Hiroyuki, Suzuki Rikako, Sugie Shigeyuki, Murakami Akira, Ohigashi Hajime, Tanaka Takuji
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Oncol Rep. 2006 May;15(5):1169-73.
The modifying effects of dietary feeding with chrysin (5,7-dihydroxyflavone) on the development of azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) were investigated in male F344 rats. We also assessed the effect of chrysin on mitosis and apoptosis in 'normal appearing' crypts. To induce ACF, rats were given two weekly subcutaneous injections of AOM (20 mg/kg body weight). They also received an experimental diet containing chrysin (0.001 or 0.01%) for 4 weeks, starting 1 week before the first dose of AOM. AOM exposure produced a substantial number of ACF (73+/-13/rat) at the end of the study (week 4). Dietary administration of chrysin caused significant reduction in the frequency of ACF: 0.001% chrysin, 37+/-17/rat (49% reduction, P<0.001); and 0.01% chrysin, 40+/-10/rat (45% reduction, P<0.001). In addition, chrysin administration significantly reduced the mitotic index and significantly increased the apoptotic index in 'normal appearing' crypts. These findings might suggest a possible chemopreventive activity of chrysin in the early step of colon tumorigenesis through modulation of cryptal cell proliferation activity and apoptosis.
在雄性F344大鼠中研究了用白杨素（5,7 - 二羟基黄酮）进行饮食喂养对氧化偶氮甲烷（AOM）诱导的结肠异常隐窝灶（ACF）发生发展的调节作用。我们还评估了白杨素对“外观正常”隐窝中细胞有丝分裂和细胞凋亡的影响。为诱导ACF，给大鼠每周皮下注射两次AOM（20 mg/kg体重）。从第一剂AOM前1周开始，它们还接受含白杨素（0.001%或0.01%）的实验饮食4周。在研究结束时（第4周），AOM暴露产生了大量的ACF（73±13/只大鼠）。饮食给予白杨素导致ACF频率显著降低：0.001%白杨素组，37±17/只大鼠（降低49%，P<0.001）；0.01%白杨素组，40±10/只大鼠（降低45%，P<0.001）。此外，给予白杨素显著降低了“外观正常”隐窝中的有丝分裂指数，并显著增加了凋亡指数。这些发现可能提示白杨素在结肠肿瘤发生的早期阶段可能通过调节隐窝细胞增殖活性和细胞凋亡而具有化学预防活性。
