印度尼西亚鼻窦淋巴瘤的基因突变分析

Gene mutation analysis of sinonasal lymphomas in Indonesia.

作者信息

Kurniawan Antonius Nikolas, Hongyo Tadashi, Hardjolukito Endang S R, Ham Maria Francisca, Takakuwa Tetsuya, Kodariah Ria, Hoshida Yoshihiko, Nomura Taisei, Aozasa Katsuyuki

机构信息

Department of Anatomical Pathology, Faculty of Medicine, University of Indonesia/Dr. Cipto Mangunkusumo Hospital, Jalan Salemba Raya, Jakarta 10430, Indonesia.

出版信息

Oncol Rep. 2006 May;15(5):1257-63.

PMID:16596195

Abstract

Sinonasal lymphomas comprise NK/T-cell (NKTCL) type and B-cell type with unique geographical development. In this study, mutations of p53, K-ras, c-kit, beta-catenin, and bak gene were analyzed using polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) followed by direct sequencing in 41 sinonasal lymphomas (27 NKTCL and 14 B-cell type) from Indonesia. In situ hybridization study with EBER-1 probe revealed that 85% of NKTCL cases were EBV positive, but none of B-cell type was EBV positive. Frequency of mutations in p53, K-ras, c-kit, beta-catenin, and bak gene was 62.9%, 0%, 11.1%, 18.5%, and 25.9%, respectively, in NKTCL, and 71.4%, 0%, 23.1%, 21.4%, and 57.1%, respectively, in B-cell cases, showing that mutation frequency in all genes was higher in B-cell than in NKTCL cases. These findings suggest that gene mutations might be the driving-force for B-cell lymphoma, whereas combined EBV infection and gene mutations contribute to NKTCL development in Indonesia.

摘要

鼻窦淋巴瘤包括NK/T细胞型（NKTCL）和B细胞型，具有独特的地域发展特征。在本研究中，采用聚合酶链反应（PCR）-单链构象多态性（SSCP）分析了来自印度尼西亚的41例鼻窦淋巴瘤（27例NKTCL和14例B细胞型）中p53、K-ras、c-kit、β-连环蛋白和bak基因的突变情况，随后进行直接测序。用EBER-1探针进行原位杂交研究显示，85%的NKTCL病例EBV呈阳性，但B细胞型均未检测到EBV阳性。在NKTCL中，p53、K-ras、c-kit、β-连环蛋白和bak基因的突变频率分别为62.9%、0%、11.1%、18.5%和25.9%，在B细胞型病例中分别为71.4%、0%、23.1%、21.4%和57.1%，表明所有基因的突变频率在B细胞型中均高于NKTCL病例。这些发现提示，基因突变可能是B细胞淋巴瘤的驱动因素，而EBV感染与基因突变共同促进了印度尼西亚NKTCL的发生发展。