Trog Daniela, Fountoulakis Michael, Friedlein Arno, Golubnitschaja Olga
Department of Radiology, Division of Molecular/Experimental Radiology, Friedrich-Wilhelms-University of Bonn, Bonn, Germany.
Proteomics. 2006 May;6(9):2924-30. doi: 10.1002/pmic.200500587.
The most common human brain tumours - gliomas - have poor prognosis with and without treatment. The current therapy conditions act sub-lethally and cannot effectively suppress the proliferation of glioma cells. Here we show differential protein expression patterns in surviving human malignant U87-MG glioma cells under clinically relevant chemo/radiotherapy. In parallel experiments, the cells underwent either irradiation (2 Gy, 200 KV X-ray) or chemotreatment with 30 microg/mL of temozolomide in the cultivation medium or combined chemo/radiation treatment. The cell cultures were treated during 5 days from day 4 until day 9 of growth. Modulated expression patterns of vimentin and RhoA GTPase indicate a potentially increasing grade of malignancy in treated cell fractions correlating well with extremely aggressive tumour phenotypes observed clinically at recidivation of treated malignant gliomas.
最常见的人类脑肿瘤——胶质瘤——无论是否接受治疗，预后都很差。目前的治疗条件只能产生亚致死效应，无法有效抑制胶质瘤细胞的增殖。在此，我们展示了在临床相关的化疗/放疗条件下，存活的人类恶性U87-MG胶质瘤细胞中的差异蛋白表达模式。在平行实验中，细胞分别接受了照射（2 Gy，200 KV X射线）、在培养基中用30μg/mL替莫唑胺进行化学处理或联合化疗/放疗。细胞培养物在生长的第4天至第9天期间接受了5天的处理。波形蛋白和RhoA GTP酶的表达模式变化表明，处理后的细胞部分中恶性程度可能增加，这与治疗后的恶性胶质瘤复发时临床上观察到的极具侵袭性的肿瘤表型密切相关。
