反相模式下的高效液相色谱法：用于研究黑加仑籽油在超临界二氧化碳中脂解动力学的工具。
HPLC in reversed phase mode: Tool for investigation of kinetics of blackcurrant seed oil lipolysis in supercritical carbon dioxide.
作者信息
Bártlová Milena, Bernásek Prokop, Sýkora Jan, Sovová Helena
机构信息
Institute of Chemical Process Fundamentals, Academy of Sciences of the Czech Republic, Rozvojová 135, 165 02 Prague 6, Czech Republic.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jul 24;839(1-2):80-4. doi: 10.1016/j.jchromb.2006.03.035. Epub 2006 Apr 4.
Blackcurrant (Ribes nigrum) seed oil is rich in alpha- and gamma-linolenic acids, the latter in particular being of potential use in medicine. The enzymatic hydrolysis of the oil was carried out in supercritical carbon dioxide using lipase Lipozyme as catalyst and changes in the composition of acylglycerols were recorded. Mono-, di-, and triacylglycerols and free fatty acids were separated by non-aqueous high-performance liquid chromatography in reversed phase mode and detected by UV diode array and 1H NMR detectors. Lipozyme was found to exert low specificity to individual fatty acids in the hydrolysed oil.
黑加仑（醋栗）籽油富含α-亚麻酸和γ-亚麻酸，后者在医学上尤其具有潜在用途。以脂肪酶Lipozyme为催化剂，在超临界二氧化碳中对该油进行酶促水解，并记录了酰基甘油组成的变化。通过反相模式的非水高效液相色谱法分离单酰基甘油、二酰基甘油、三酰基甘油和游离脂肪酸，并通过紫外二极管阵列和1H NMR检测器进行检测。结果发现，Lipozyme对水解油中的单个脂肪酸具有较低的特异性。
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